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Literature summary extracted from
- Purification and characterization of the gamma regulatory subunit of the cyclic GMP phosphodiesterase from retinal rod outer segments (1982), J. Biol. Chem., 257, 11094-11099.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.35 | light | activation | Bos taurus | |
| 3.1.4.35 | Trypsin | - |
Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.35 | Inhibitor protein | gamma-subunit of enzyme | Bos taurus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.35 | 0.078 | - |
3',5'-cGMP | trypsin activated enzyme | Bos taurus |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.4.35 | photoreceptor outer segment | rod outer segment | Bos taurus | 1750 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.35 | 88000 | - |
88000, alpha-subunit, + 84000, beta-subunit, + 1100, gamma-subunit, inhibitory, SDS-PAGE | Bos taurus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.35 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.35 | - |
Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.35 | retina | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.35 | 20 | 100 | unactivated purified enzyme | Bos taurus |
| 3.1.4.35 | 1200 | 1500 | trypsin-activated enzyme | Bos taurus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.4.35 | -20°C, 10 mM morpholinopropanesulfonic acid, 1.5 mM MgCl2, 0.1 mM phenylmethylsulfonyl fluoride, 1 mM dithiothreitol, 50% glycerol, pH 7.5, more than 6 months | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.35 | 3',5'-cGMP + H2O | - |
Bos taurus | 5'-GMP | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.4.35 | ? | 88000, alpha-subunit, + 84000, beta-subunit, + 1100, gamma-subunit, inhibitory, SDS-PAGE | Bos taurus |
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