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Literature summary extracted from

  • Hansen, S.; Hough, E.; Svensson, L.A.; Wong, Y.L.; Martin, S.F.
    Crystal structure of phospholipase C from Bacillus cereus complexed with a substrate analogue (1993), J. Mol. Biol., 234, 179-189.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.3 1.9 A resolution, R-factor of 15.7% Bacillus cereus
3.1.4.3 crystal structure of a complex between the enzyme and a competitive inhibitor. 1.9 A resolution, R-factor of 15.7% Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.3 3(S),4-dihexanoylbutyl-1-phosphonycholine non-hydrolyzable, competitive inhibitor of bacterial PLC, analog of the natural phospholipid substrate, binds with its phosphonyl group to the three Zn-ions in the active site of the enzyme Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.3 0.38
-
 phosphatidylcholine water-soluble substrate Bacillus cereus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.3 extracellular
-
 Bacillus cereus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.3 Zn2+ metalloenzyme Bacillus cereus
3.1.4.3 Zn2+ 3 Zn2+ ions in active site Bacillus cereus
3.1.4.3 Zn2+ in the active site of the enzyme Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Bacillus cereus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3
-
 Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 phosphatidylcholine + H2O
-
 Bacillus cereus 1,2-diacylglycerol + choline phosphate
-
 ?
3.1.4.3 phosphatidylethanolamine + H2O
-
 Bacillus cereus phosphorylethanolamine + diacylglycerol
-
 ?
3.1.4.3 phosphatidylserine + H2O
-
 Bacillus cereus phosphorylserine + diacylglycerol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.3 PC-PLC
-
 Bacillus cereus
3.1.4.3 phospholipase C
-
 Bacillus cereus
3.1.4.3 PLC
-
 Bacillus cereus
3.1.4.3 PLCBC
-
 Bacillus cereus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.4.3 0.47
-
 3(S),4-dihexanoylbutyl-1-phosphonycholine
-
 Bacillus cereus