BRENDA - Enzyme Database

Studies on phospholipases from Streptomyces. Purification and properties of Streptomyces hachijoensis phospholipase C

Okawa, Y.; Yamaguchi, T.; J. Biochem. 78, 537-545 (1975)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
ethyl ether
C-1
Streptomyces hachijoensis
General Stability
EC Number
General Stability
Organism
3.1.4.3
sodium dodecyl sulfate
Streptomyces hachijoensis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
Al3+
C-1
Streptomyces hachijoensis
3.1.4.3
Ba2+
C-1
Streptomyces hachijoensis
3.1.4.3
Ca2+
C-1
Streptomyces hachijoensis
3.1.4.3
Cu2+
C-1
Streptomyces hachijoensis
3.1.4.3
EDTA
C-1
Streptomyces hachijoensis
3.1.4.3
Fe2+
C-1
Streptomyces hachijoensis
3.1.4.3
Fe3+
C-1
Streptomyces hachijoensis
3.1.4.3
SDS
C-1
Streptomyces hachijoensis
3.1.4.3
sodium cholate
C-1, at concentrations above 0.02%
Streptomyces hachijoensis
3.1.4.3
sodium deoxycholate
C-1, at concentrations above 0.02%
Streptomyces hachijoensis
3.1.4.3
sodium lauryl sulfate
C-1, at concentrations above 0.02%
Streptomyces hachijoensis
3.1.4.3
Triton X-100
C-1, greater 0.02%
Streptomyces hachijoensis
3.1.4.3
Zn2+
C-1
Streptomyces hachijoensis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.3
0.9
-
phosphatidylcholine
C-1
Streptomyces hachijoensis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Streptomyces hachijoensis
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Mg2+
-
Streptomyces hachijoensis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
18000
-
gel filtration, C-1 and C-2
Streptomyces hachijoensis
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
3.1.4.3
ethyl alcohol
-
Streptomyces hachijoensis
3.1.4.3
ethyl ether
C-1 activating at a concentration of 20% v/v
Streptomyces hachijoensis
3.1.4.3
ethyl ether-ethanol
-
Streptomyces hachijoensis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.3
Streptomyces hachijoensis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.3
C-1and C-2: dialysis, isoelectric focusing and column chromatography, C-1: 1510foldfold, C-2: 1330fold, both to homogeneity
Streptomyces hachijoensis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.1.4.3
74.3
-
C-2, substrate: lecithin
Streptomyces hachijoensis
3.1.4.3
84.6
-
C-1, substrate: lecithin
Streptomyces hachijoensis
Storage Stability
EC Number
Storage Stability
Organism
3.1.4.3
4C, lyophilized crude preparation, stable for at least 6 months
Streptomyces hachijoensis
3.1.4.3
frozen, neutral pH, almost full activity after 2 years
Streptomyces hachijoensis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
cardiolipin + H2O
-
135307
Streptomyces hachijoensis
phosphatidylglycerophosphate + 1,2-diacylglycerol
-
135307
Streptomyces hachijoensis
?
3.1.4.3
lysophosphatidylcholine + H2O
-
135307
Streptomyces hachijoensis
monoacylglycerol + phosphorylcholine
-
135307
Streptomyces hachijoensis
?
3.1.4.3
phosphatidic acid + H2O
-
135307
Streptomyces hachijoensis
?
-
-
-
?
3.1.4.3
phosphatidylcholine + H2O
-
135307
Streptomyces hachijoensis
1,2-diacylglycerol + phosphorylcholine
-
135307
Streptomyces hachijoensis
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135307
Streptomyces hachijoensis
phosphorylethanolamine + diacylglycerol
-
135307
Streptomyces hachijoensis
?
3.1.4.3
phosphatidylinositol + H2O
-
135307
Streptomyces hachijoensis
inositol monophosphate + diacylglycerol
-
135307
Streptomyces hachijoensis
?
3.1.4.3
phosphatidylserine + H2O
-
135307
Streptomyces hachijoensis
phosphorylserine + diacylglycerol
-
135307
Streptomyces hachijoensis
?
3.1.4.3
sphingomyelin + H2O
-
135307
Streptomyces hachijoensis
ceramide + phosphorylcholine
-
135307
Streptomyces hachijoensis
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.4.3
50
-
C-1
Streptomyces hachijoensis
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.1.4.3
20
-
pH values between 5.0-9.0, stable for 2 hours, pH values between 6.0-7.0, 95% of activity remains after 24 hours
Streptomyces hachijoensis
3.1.4.3
37
-
for 90 min, 75% of activity remains
Streptomyces hachijoensis
3.1.4.3
50
-
C-1, for 30 min, stable
Streptomyces hachijoensis
3.1.4.3
70
-
complete loss of activity
Streptomyces hachijoensis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
8
-
C-1, substrate: lecithin
Streptomyces hachijoensis
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.4.3
7
-
C-1, stable
Streptomyces hachijoensis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.3
ethyl ether
C-1
Streptomyces hachijoensis
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.4.3
sodium dodecyl sulfate
Streptomyces hachijoensis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.3
Al3+
C-1
Streptomyces hachijoensis
3.1.4.3
Ba2+
C-1
Streptomyces hachijoensis
3.1.4.3
Ca2+
C-1
Streptomyces hachijoensis
3.1.4.3
Cu2+
C-1
Streptomyces hachijoensis
3.1.4.3
EDTA
C-1
Streptomyces hachijoensis
3.1.4.3
Fe2+
C-1
Streptomyces hachijoensis
3.1.4.3
Fe3+
C-1
Streptomyces hachijoensis
3.1.4.3
SDS
C-1
Streptomyces hachijoensis
3.1.4.3
sodium cholate
C-1, at concentrations above 0.02%
Streptomyces hachijoensis
3.1.4.3
sodium deoxycholate
C-1, at concentrations above 0.02%
Streptomyces hachijoensis
3.1.4.3
sodium lauryl sulfate
C-1, at concentrations above 0.02%
Streptomyces hachijoensis
3.1.4.3
Triton X-100
C-1, greater 0.02%
Streptomyces hachijoensis
3.1.4.3
Zn2+
C-1
Streptomyces hachijoensis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.3
0.9
-
phosphatidylcholine
C-1
Streptomyces hachijoensis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.3
extracellular
-
Streptomyces hachijoensis
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.3
Mg2+
-
Streptomyces hachijoensis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.3
18000
-
gel filtration, C-1 and C-2
Streptomyces hachijoensis
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
3.1.4.3
ethyl alcohol
-
Streptomyces hachijoensis
3.1.4.3
ethyl ether
C-1 activating at a concentration of 20% v/v
Streptomyces hachijoensis
3.1.4.3
ethyl ether-ethanol
-
Streptomyces hachijoensis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.3
C-1and C-2: dialysis, isoelectric focusing and column chromatography, C-1: 1510foldfold, C-2: 1330fold, both to homogeneity
Streptomyces hachijoensis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.1.4.3
74.3
-
C-2, substrate: lecithin
Streptomyces hachijoensis
3.1.4.3
84.6
-
C-1, substrate: lecithin
Streptomyces hachijoensis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.4.3
4C, lyophilized crude preparation, stable for at least 6 months
Streptomyces hachijoensis
3.1.4.3
frozen, neutral pH, almost full activity after 2 years
Streptomyces hachijoensis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.3
cardiolipin + H2O
-
135307
Streptomyces hachijoensis
phosphatidylglycerophosphate + 1,2-diacylglycerol
-
135307
Streptomyces hachijoensis
?
3.1.4.3
lysophosphatidylcholine + H2O
-
135307
Streptomyces hachijoensis
monoacylglycerol + phosphorylcholine
-
135307
Streptomyces hachijoensis
?
3.1.4.3
phosphatidic acid + H2O
-
135307
Streptomyces hachijoensis
?
-
-
-
?
3.1.4.3
phosphatidylcholine + H2O
-
135307
Streptomyces hachijoensis
1,2-diacylglycerol + phosphorylcholine
-
135307
Streptomyces hachijoensis
?
3.1.4.3
phosphatidylethanolamine + H2O
-
135307
Streptomyces hachijoensis
phosphorylethanolamine + diacylglycerol
-
135307
Streptomyces hachijoensis
?
3.1.4.3
phosphatidylinositol + H2O
-
135307
Streptomyces hachijoensis
inositol monophosphate + diacylglycerol
-
135307
Streptomyces hachijoensis
?
3.1.4.3
phosphatidylserine + H2O
-
135307
Streptomyces hachijoensis
phosphorylserine + diacylglycerol
-
135307
Streptomyces hachijoensis
?
3.1.4.3
sphingomyelin + H2O
-
135307
Streptomyces hachijoensis
ceramide + phosphorylcholine
-
135307
Streptomyces hachijoensis
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.4.3
50
-
C-1
Streptomyces hachijoensis
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.1.4.3
20
-
pH values between 5.0-9.0, stable for 2 hours, pH values between 6.0-7.0, 95% of activity remains after 24 hours
Streptomyces hachijoensis
3.1.4.3
37
-
for 90 min, 75% of activity remains
Streptomyces hachijoensis
3.1.4.3
50
-
C-1, for 30 min, stable
Streptomyces hachijoensis
3.1.4.3
70
-
complete loss of activity
Streptomyces hachijoensis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.3
8
-
C-1, substrate: lecithin
Streptomyces hachijoensis
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.4.3
7
-
C-1, stable
Streptomyces hachijoensis