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Literature summary extracted from

  • Otnaess, A.B.; Little, C.; Sletten, K.; Wallin, R.; Johnsen, S.; Flengsrud, R.; Prydz, H.
    Some characteristics of phospholipase C from Bacillus cereus (1977), Eur. J. Biochem., 79, 459-468.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.3 sodium deoxycholate 0.1% Bacillus cereus

Application

EC Number Application Comment Organism
3.1.4.3 medicine inactivation of thromboplastin, potential therapeutic value in cases of intravascular coagulation Bacillus cereus

General Stability

EC Number General Stability Organism
3.1.4.3 1,10-phenanthroline, denaturation Bacillus cereus
3.1.4.3 guanidine HCl, stable Bacillus cereus
3.1.4.3 sodium dodecyl sulfate Bacillus cereus
3.1.4.3 sodium dodecyl sulfate, denaturation Bacillus cereus
3.1.4.3 sodium dodecyl sulfate, enzyme resistant to denaturation in presence of 1 mM Zn2+ Bacillus cereus
3.1.4.3 urea, enzyme stable in presence of 1 mM Zn2+ Bacillus cereus
3.1.4.3 urea, PLC is catalytically active in 8 M urea Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.3 EDTA
-
 Bacillus cereus
3.1.4.3 o-phenanthroline denaturation Bacillus cereus
3.1.4.3 SDS
-
 Bacillus cereus
3.1.4.3 Urea denaturation; denaturation only in absence of free Zn2+ Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.3 0.6
-
 1,2-dipalmitoylphosphatidylcholine
-
 Bacillus cereus
3.1.4.3 2 3 phosphatidylserine
-
 Bacillus cereus
3.1.4.3 4.5 7 1,2-dipalmitoylphosphatidylethanolamine
-
 Bacillus cereus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.3 extracellular
-
 Bacillus cereus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.3 Zn2+ metalloenzyme Bacillus cereus
3.1.4.3 Zn2+ 2 mol Zn2+ per mol enzyme Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.3 23000
-
-
 Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Bacillus cereus
-
 strain ATCC 10987 AB-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3
-
 Bacillus cereus

Storage Stability

EC Number Storage Stability Organism
3.1.4.3 -20°C, in barbital-buffered saline, pH 7.4, 1 mM ZnSO4, stable for more than one year Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 1,2-dicaproylphosphatidylcholine + H2O
-
 Bacillus cereus 1,2-dicaproylglycerol + phosphorylcholine
-
 ?
3.1.4.3 1,2-dimyristoylphosphatidylcholine + H2O
-
 Bacillus cereus 1,2-dimyristoylglycerol + phosphorylcholine
-
 ?
3.1.4.3 1,2-dipalmitoylphosphatidylcholine + H2O
-
 Bacillus cereus 1,2-dipalmitoylglycerol + phosphorylcholine
-
 ?
3.1.4.3 1,2-dipalmitoylphosphatidylethanolamine + H2O
-
 Bacillus cereus 1,2-dipalmitoylglycerol + phosphorylethanolamine
-
 ?
3.1.4.3 phosphatidylcholine + H2O
-
 Bacillus cereus 1,2-diacylglycerol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylethanolamine + H2O
-
 Bacillus cereus phosphorylethanolamine + diacylglycerol
-
 ?
3.1.4.3 phosphatidylserine + H2O
-
 Bacillus cereus phosphorylserine + diacylglycerol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.3 monomer 23000, no comment on method Bacillus cereus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.3 35 60 substrate: dimyristoylphosphatidylcholine Bacillus cereus
3.1.4.3 47
-
 substrate: dipalmitoylphosphatidylcholine Bacillus cereus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.4.3 10 76
-
 Bacillus cereus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.3 76
-
 for 6 min, with 0.1 mM free Zn2+: loss of 21% of activity, without Zn2+: loss of 63% of activity Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.3 8
-
 substrate: dicaproyl phosphatidylcholine Bacillus cereus
3.1.4.3 8 8.3 subatrate: dipalmitoylphosphatidylcholine Bacillus cereus