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Literature summary extracted from

  • Yamakawa, Y.; Ohsaka, A.
    Purification and some properties of phospholipase C (a-toxin) of Clostridium perfringens (1977), J. Biochem., 81, 115-126.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.3 sodium cholate
-
 Clostridium perfringens
3.1.4.3 sodium dehydrocholate
-
 Clostridium perfringens
3.1.4.3 sodium deoxycholate at concentrations up to 0.83mg/ml, optimal molar ratio of sodium deoxycholate to lecithin: 0.5 Clostridium perfringens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.3 sodium deoxycholate at concentrations above 2.5 mg/ml Clostridium perfringens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.3 extracellular
-
 Clostridium perfringens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.3 Ca2+
-
 Clostridium perfringens
3.1.4.3 Co2+
-
 Clostridium perfringens
3.1.4.3 Mn2+
-
 Clostridium perfringens
3.1.4.3 Zn2+
-
 Clostridium perfringens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.3 31000
-
 gel filtration Clostridium perfringens
3.1.4.3 43000
-
 SDS-PAGE Clostridium perfringens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.3 phosphatidylcholine + H2O Clostridium perfringens
-
 1,2-diacylglycerol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylcholine + H2O Clostridium perfringens PB6K
-
 1,2-diacylglycerol + phosphorylcholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Clostridium perfringens
-
-
-
3.1.4.3 Clostridium perfringens PB6K
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3 column chromatography, gel filtration Clostridium perfringens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.3 1575
-
 substrate: phosphatidylcholine Clostridium perfringens

Storage Stability

EC Number Storage Stability Organism
3.1.4.3 -20°C, crude enzyme, stable for more than 6 months Clostridium perfringens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 phosphatidylcholine + H2O
-
 Clostridium perfringens 1,2-diacylglycerol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylcholine + H2O
-
 Clostridium perfringens PB6K 1,2-diacylglycerol + phosphorylcholine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.3 monomer 31000-43000, gel filtration, SDS-PAGE Clostridium perfringens
3.1.4.3 polymer identical subunits of 31000-43000 Da, gel filtration, SDS-PAGE, multiple forms Clostridium perfringens