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Literature summary extracted from

  • Meij, J.T.A.; Ross, E.T.
    Purification and characterization of phospholipase C-¯1 mutants expressed in E.coli (1996), Biochem. Biophys. Res. Commun., 225, 705-711.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.3 subunit of cell surface receptor associated G-protein Gqa, subunit of cell surface receptor associated G-protein, PLC d1 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.3 tranformed in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.3 G758D
-
 Homo sapiens
3.1.4.3 S541Y
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.3 150000
-
 PLC beta 1, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.3 phosphatidylinositol-bisphosphate + H2O Homo sapiens
-
 inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3 recombinant PLC, NiNTA, two FPLC steps, 160fold Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.3 1050000
-
 substrate: phosphatidylinositol-4,5-bisphosphate Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.1.4.3 -80°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 phosphatidylinositol-bisphosphate + H2O
-
 Homo sapiens inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
 ?