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Literature summary extracted from

  • Lim, S.T.; Palanisamy, U.M.; Ong, K.K.
    Purification and properties of 3',5'-cyclic nucleotide phosphodiesterase from Rhizobium fredii MAR-1 (1986), Arch. Microbiol., 146, 142-146.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.17 ascorbic acid reduced, slight stimulation Sinorhizobium fredii
3.1.4.17 glutathione reduced, slight stimulation Sinorhizobium fredii
3.1.4.17 imidazole
-
 Sinorhizobium fredii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.17 Al3+
-
 Sinorhizobium fredii
3.1.4.17 Caffeine
-
 Sinorhizobium fredii
3.1.4.17 dibutyryl cyclic AMP 94% inhibition at 2.5 mM Sinorhizobium fredii
3.1.4.17 FAD
-
 Sinorhizobium fredii
3.1.4.17 Fe2+
-
 Sinorhizobium fredii
3.1.4.17 Fe3+
-
 Sinorhizobium fredii
3.1.4.17 methylxanthines
-
 Sinorhizobium fredii
3.1.4.17 NAD+
-
 Sinorhizobium fredii
3.1.4.17 NADP+
-
 Sinorhizobium fredii
3.1.4.17 theophylline
-
 Sinorhizobium fredii
3.1.4.17 Zn2+
-
 Sinorhizobium fredii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.17 additional information
-
 additional information
-
 Sinorhizobium fredii
3.1.4.17 0.0001
-
 3',5'-cAMP
-
 Sinorhizobium fredii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.17 Cu2+ stimulates at 2.5 mM Sinorhizobium fredii
3.1.4.17 additional information no metal ion required Sinorhizobium fredii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.17 57000
-
 gel filtration Sinorhizobium fredii
3.1.4.17 59000
-
 SDS-PAGE Sinorhizobium fredii

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.17 Sinorhizobium fredii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.17
-
 Sinorhizobium fredii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.17 0.727
-
-
 Sinorhizobium fredii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.17 3',5'-cAMP + H2O
-
 Sinorhizobium fredii 5'-AMP
-
 ?
3.1.4.17 3',5'-cGMP + H2O
-
 Sinorhizobium fredii 5'-GMP
-
 ?
3.1.4.17 additional information not: 2',3'-cAMP Sinorhizobium fredii ?
-
 ?
3.1.4.17 nucleoside 3',5'-cyclic phosphate + H2O
-
 Sinorhizobium fredii nucleoside 5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.17 monomer 1 * 59000, SDS-PAGE Sinorhizobium fredii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.17 28
-
 80% of activity remains for at least 10 days Sinorhizobium fredii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.17 7
-
-
 Sinorhizobium fredii