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Literature summary extracted from

  • Lim, S.T.; Krishnan, A.K.; Ong, K.K.
    Purification and characterisation of the second form of cyclic 3',5'-nucleotide phosphodiesterase from Rhizobium fredii (1989), Biochim. Biophys. Acta, 991, 353-358.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.17 2-mercaptoethanol stimulates Sinorhizobium fredii
3.1.4.17 ascorbic acid stimulates Sinorhizobium fredii
3.1.4.17 dithiothreitol stimulates Sinorhizobium fredii
3.1.4.17 glutathione reduced, stimulates Sinorhizobium fredii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.17 Al3+ strongly inhibitory at 2.5 mM Sinorhizobium fredii
3.1.4.17 Fe2+ strongly inhibitory at 2.5 mM Sinorhizobium fredii
3.1.4.17 Fe3+ strongly inhibitory at 2.5 mM Sinorhizobium fredii
3.1.4.17 Zn2+ strongly inhibitory at 2.5 mM Sinorhizobium fredii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.17 0.0018
-
 3',5'-cAMP
-
 Sinorhizobium fredii
3.1.4.17 0.002
-
 3',5'-cGMP
-
 Sinorhizobium fredii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.17 Ba2+ 40% stimulation at 0.25 mM Sinorhizobium fredii
3.1.4.17 Ca2+ 40% stimulation at 0.25 mM Sinorhizobium fredii
3.1.4.17 Co2+ stimulates at 2.5 mM Sinorhizobium fredii
3.1.4.17 Mg2+ stimulates at 2.5 mM Sinorhizobium fredii
3.1.4.17 Mn2+ stimulates at 2.5 mM Sinorhizobium fredii
3.1.4.17 additional information no metal ion required Sinorhizobium fredii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.17 28000
-
 x * 28000 Sinorhizobium fredii
3.1.4.17 82000
-
 Rhizobium fredii MAR-1, gel filtration Sinorhizobium fredii

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.17 Sinorhizobium fredii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.17 95.7fold Sinorhizobium fredii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.17 0.096
-
-
 Sinorhizobium fredii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.17 3',5'-cAMP + H2O
-
 Sinorhizobium fredii 5'-AMP
-
 ?
3.1.4.17 3',5'-cGMP + H2O
-
 Sinorhizobium fredii 5'-GMP
-
 ?
3.1.4.17 nucleoside 3',5'-cyclic phosphate + H2O
-
 Sinorhizobium fredii nucleoside 5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.17 oligomer SDS-PAGE Sinorhizobium fredii
3.1.4.17 oligomer x * 28000 Sinorhizobium fredii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.17 28
-
 80% of activity remaining after 7 days Sinorhizobium fredii
3.1.4.17 60
-
 20 min, no loss of activity Sinorhizobium fredii
3.1.4.17 70
-
 20 min, 20% loss of activity Sinorhizobium fredii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.17 7 7.5
-
 Sinorhizobium fredii