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Literature summary extracted from

  • Anraku, Y.
    A new cyclic phosphodiesterase having a 3'-nucleotidase activity from Escherichia coli B. I. PURIFICATION AND SOME PROPERTIES OF THE ENZYME. (1964), J. Biol. Chem., 239, 3412-3419.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.16 Zn2+
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.16 additional information
-
 additional information
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.16 Co2+ activates Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.16 Escherichia coli
-
-
-
3.1.4.16 Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.16
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.16 additional information
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.1.4.16 2-4°C, pH 7.4-7.6, Tris buffer, 20-30% loss of activity after 2 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.16 cyclic 2',3'-AMP + H2O
-
 Escherichia coli 3'-AMP
-
 ?
3.1.4.16 cyclic 2',3'-AMP + H2O
-
 Escherichia coli B / ATCC 11303 3'-AMP
-
 ?
3.1.4.16 cyclic 2',3'-CMP + H2O
-
 Escherichia coli 3'-CMP
-
 ?
3.1.4.16 cyclic 2',3'-CMP + H2O
-
 Escherichia coli B / ATCC 11303 3'-CMP
-
 ?
3.1.4.16 cyclic 2',3'-GMP + H2O
-
 Escherichia coli 3'-GMP
-
 ?
3.1.4.16 cyclic 2',3'-GMP + H2O
-
 Escherichia coli B / ATCC 11303 3'-GMP
-
 ?
3.1.4.16 cyclic 2',3'-UMP + H2O
-
 Escherichia coli 3'-UMP
-
 ?
3.1.4.16 cyclic 2',3'-UMP + H2O
-
 Escherichia coli B / ATCC 11303 3'-UMP
-
 ?
3.1.4.16 di-p-nitrophenyl phosphate + H2O
-
 Escherichia coli p-nitrophenol + p-nitrophenyl phosphate
-
 ?
3.1.4.16 di-p-nitrophenyl phosphate + H2O
-
 Escherichia coli B / ATCC 11303 p-nitrophenol + p-nitrophenyl phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.16 55
-
 inactivation Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.16 5 6.5 hydrolysis of di-p-nitrophenyl phosphate Escherichia coli
3.1.4.16 6.4
-
 3'-nucleotidase activity and cyclic phosphodiesterase activity against cyclic 2',3'-UMP Escherichia coli