EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.16 | Br- | activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate | Vibrio alginolyticus | |
3.1.4.16 | CH3COO- | activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate | Vibrio alginolyticus | |
3.1.4.16 | Cl- | activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate | Vibrio alginolyticus | |
3.1.4.16 | HCOO- | activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate | Vibrio alginolyticus | |
3.1.4.16 | I- | activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate | Vibrio alginolyticus | |
3.1.4.16 | additional information | monovalent cations activate in the order of decreasing effectiveness: Cl-, Br-/I-, NO3-, HCOO-, CH3COO- | Vibrio alginolyticus | |
3.1.4.16 | NO3- | activates hydrolysis of 3'-nucleotides and di-p-dinitrophenyl phosphate | Vibrio alginolyticus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.16 | Cd2+ | 0.2 mM, 30% inhibition of hydrolysis of di-p-nitrophenyl phosphate, 20% inhibition of 3'-AMP hydrolysis | Vibrio alginolyticus | |
3.1.4.16 | Hg2+ | 0.2 mM, 35% inhibition of hydrolysis of di-p-nitrophenyl phosphate, 36% inhibition of 3'-AMP hydrolysis | Vibrio alginolyticus | |
3.1.4.16 | MgCl2 | maximal inhibition at 20 mM | Vibrio alginolyticus | |
3.1.4.16 | MgSO4 | slight inhibition | Vibrio alginolyticus | |
3.1.4.16 | Na2SO4 | slight inhibition | Vibrio alginolyticus | |
3.1.4.16 | Zn2+ | 0.2 mM, 90% inhibition of hydrolysis of di-p-nitrophenyl phosphate, 78% inhibition of 3'-AMP hydrolysis | Vibrio alginolyticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.16 | 0.01 | - |
3'-CMP | - |
Vibrio alginolyticus | |
3.1.4.16 | 0.05 | - |
3'-UMP | - |
Vibrio alginolyticus | |
3.1.4.16 | 0.08 | - |
3'-AMP | - |
Vibrio alginolyticus | |
3.1.4.16 | 0.1 | - |
3'-GMP | - |
Vibrio alginolyticus | |
3.1.4.16 | 0.1 | - |
cyclic 2',3'-CMP | - |
Vibrio alginolyticus | |
3.1.4.16 | 0.2 | - |
di-p-nitrophenyl phosphate | with 200 mM NaCl | Vibrio alginolyticus | |
3.1.4.16 | 0.36 | - |
cyclic 2',3'-UMP | - |
Vibrio alginolyticus | |
3.1.4.16 | 0.45 | - |
di-p-nitrophenyl phosphate | without NaCl | Vibrio alginolyticus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.4.16 | additional information | results might indicate a surface localization | Vibrio alginolyticus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.16 | Mn2+ | 0.2 mM, 6-10% stimulation | Vibrio alginolyticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.4.16 | 65000 | - |
gel filtration | Vibrio alginolyticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.16 | Vibrio alginolyticus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.4.16 | - |
Vibrio alginolyticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.4.16 | additional information | - |
- |
Vibrio alginolyticus |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.4.16 | -20°C, pH 8.0, 20 mM Tris buffer, stable for at least 6 months | Vibrio alginolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.16 | 3'-AMP + H2O | - |
Vibrio alginolyticus | adenosine + phosphate | - |
? | |
3.1.4.16 | 3'-CMP + H2O | - |
Vibrio alginolyticus | cytidine + phosphate | - |
? | |
3.1.4.16 | 3'-GMP + H2O | - |
Vibrio alginolyticus | guanosine + phosphate | - |
? | |
3.1.4.16 | 3'-UMP + H2O | - |
Vibrio alginolyticus | uridine + phosphate | - |
? | |
3.1.4.16 | cyclic 2',3'-CMP + H2O | - |
Vibrio alginolyticus | 3'-CMP | - |
? | |
3.1.4.16 | cyclic 2',3'-GMP + H2O | - |
Vibrio alginolyticus | 3'-GMP | - |
? | |
3.1.4.16 | cyclic 2',3'-UMP + H2O | - |
Vibrio alginolyticus | 3'-UMP | - |
? | |
3.1.4.16 | di-p-nitrophenyl phosphate + H2O | - |
Vibrio alginolyticus | p-nitrophenol + p-nitrophenyl phosphate | - |
? | |
3.1.4.16 | additional information | - |
Vibrio alginolyticus | ? | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.4.16 | 55 | - |
5 min, in absence of salts, 55% loss of activity, in presence of 0.2 mM MnCl2 13% loss of activity | Vibrio alginolyticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.16 | 7.6 | - |
hydrolysis of 3'-UMP | Vibrio alginolyticus |
3.1.4.16 | 7.8 | - |
hydrolysis of 3'-CMP | Vibrio alginolyticus |
3.1.4.16 | 8.1 | - |
hydrolysis of 3'-GMP | Vibrio alginolyticus |
3.1.4.16 | 8.3 | - |
hydrolysis of 3'-AMP or di-p-nitrophenyl phosphate | Vibrio alginolyticus |
3.1.4.16 | 8.4 | - |
cyclic phosphodiesterase activity with cyclic 2',3'-phosphate | Vibrio alginolyticus |
3.1.4.16 | 8.6 | - |
cyclic phosphodiesterase activity with cyclic 2',3'-phosphate | Vibrio alginolyticus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.16 | 7.2 | 9 | pH 7.2: about 45% of maximal activity, pH 9.0: about 70% of maximal activity, hydrolysis of 3'-GMP | Vibrio alginolyticus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.16 | 5 | - |
37°C, loss of activity below | Vibrio alginolyticus |
3.1.4.16 | 6 | - |
stable for at least 2 h at 37°C | Vibrio alginolyticus |