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Literature summary extracted from

  • Drummond, G.I.; Yamamoto, M.
    Nucleoside cyclic phosphate diesterases (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 355-371.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.16 Cl- activates 3'-nucleotidase activity Vibrio alginolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.16 Hg2+ 0.2 mM Vibrio alginolyticus
3.1.4.16 Zn2+ 1 mM Bacillus subtilis
3.1.4.16 Zn2+ 10 mM Enterobacter sp.
3.1.4.16 Zn2+ 1 mM Escherichia coli
3.1.4.16 Zn2+ 0.1 and 1 mM Proteus mirabilis
3.1.4.16 Zn2+ 0.2 mM Vibrio alginolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.16 0.02
-
cyclic 2',3'-UMP
-
Shigella sonnei
3.1.4.16 0.022
-
3'-AMP
-
Proteus mirabilis
3.1.4.16 0.022
-
3'-UMP
-
Shigella sonnei
3.1.4.16 0.04
-
3'-AMP
-
Bacillus subtilis
3.1.4.16 0.045
-
3'-AMP
-
Shigella sonnei
3.1.4.16 0.05
-
3'-AMP
-
Escherichia coli
3.1.4.16 0.05
-
3'-AMP
-
Proteus vulgaris
3.1.4.16 0.05
-
3'-UMP
-
Vibrio alginolyticus
3.1.4.16 0.053
-
3'-UMP
-
Escherichia coli
3.1.4.16 0.07
-
3'-UMP
-
Proteus vulgaris
3.1.4.16 0.075
-
cyclic 2',3'-UMP
-
Proteus mirabilis
3.1.4.16 0.08
-
3'-AMP
-
Vibrio alginolyticus
3.1.4.16 0.094
-
bis(p-nitrophenyl)phosphate
-
Proteus mirabilis
3.1.4.16 0.28
-
bis(p-nitrophenyl)phosphate
-
Escherichia coli
3.1.4.16 0.36
-
cyclic 2',3'-UMP
-
Vibrio alginolyticus
3.1.4.16 0.45
-
di-p-nitrophenyl phosphate
-
Vibrio alginolyticus
3.1.4.16 0.67
-
cyclic 2',3'-UMP
-
Proteus vulgaris
3.1.4.16 0.7
-
bis(p-nitrophenyl)phosphate
-
Shigella sonnei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.16 Co2+ no activation Bacillus subtilis
3.1.4.16 Co2+ no activation Vibrio alginolyticus
3.1.4.16 Co2+ 0.1-1 mM, 3-5fold activation Escherichia coli
3.1.4.16 Co2+ 10 mM, 2-3fold stimulation Enterobacter sp.
3.1.4.16 Mn2+
-
Vibrio alginolyticus
3.1.4.16 Mn2+ 10 mM, 2-3fold stimulation Enterobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.16 Bacillus subtilis
-
-
-
3.1.4.16 Enterobacter sp.
-
-
-
3.1.4.16 Escherichia coli
-
-
-
3.1.4.16 Escherichia coli B / ATCC 11303
-
-
-
3.1.4.16 Proteus mirabilis
-
-
-
3.1.4.16 Proteus vulgaris
-
-
-
3.1.4.16 Salmonella enterica subsp. enterica serovar Heidelberg
-
-
-
3.1.4.16 Serratia marcescens
-
-
-
3.1.4.16 Shigella sonnei
-
-
-
3.1.4.16 Vibrio alginolyticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.16 3'-AMP + H2O
-
Bacillus subtilis adenosine + phosphate
-
?
3.1.4.16 3'-AMP + H2O
-
Escherichia coli adenosine + phosphate
-
?
3.1.4.16 3'-AMP + H2O
-
Proteus mirabilis adenosine + phosphate
-
?
3.1.4.16 3'-AMP + H2O
-
Proteus vulgaris adenosine + phosphate
-
?
3.1.4.16 3'-AMP + H2O
-
Vibrio alginolyticus adenosine + phosphate
-
?
3.1.4.16 3'-AMP + H2O
-
Shigella sonnei adenosine + phosphate
-
?
3.1.4.16 3'-AMP + H2O
-
Escherichia coli B / ATCC 11303 adenosine + phosphate
-
?
3.1.4.16 3'-CMP + H2O
-
Bacillus subtilis cytidine + phosphate
-
?
3.1.4.16 3'-CMP + H2O
-
Escherichia coli cytidine + phosphate
-
?
3.1.4.16 3'-CMP + H2O
-
Proteus mirabilis cytidine + phosphate
-
?
3.1.4.16 3'-CMP + H2O
-
Proteus vulgaris cytidine + phosphate
-
?
3.1.4.16 3'-CMP + H2O
-
Vibrio alginolyticus cytidine + phosphate
-
?
3.1.4.16 3'-CMP + H2O
-
Shigella sonnei cytidine + phosphate
-
?
3.1.4.16 3'-CMP + H2O
-
Escherichia coli B / ATCC 11303 cytidine + phosphate
-
?
3.1.4.16 3'-GMP + H2O
-
Bacillus subtilis guanosine + phosphate
-
?
3.1.4.16 3'-GMP + H2O
-
Escherichia coli guanosine + phosphate
-
?
3.1.4.16 3'-GMP + H2O
-
Proteus mirabilis guanosine + phosphate
-
?
3.1.4.16 3'-GMP + H2O
-
Proteus vulgaris guanosine + phosphate
-
?
3.1.4.16 3'-GMP + H2O
-
Vibrio alginolyticus guanosine + phosphate
-
?
3.1.4.16 3'-GMP + H2O
-
Shigella sonnei guanosine + phosphate
-
?
3.1.4.16 3'-GMP + H2O
-
Escherichia coli B / ATCC 11303 guanosine + phosphate
-
?
3.1.4.16 3'-UMP + H2O
-
Bacillus subtilis uridine + phosphate
-
?
3.1.4.16 3'-UMP + H2O
-
Escherichia coli uridine + phosphate
-
?
3.1.4.16 3'-UMP + H2O
-
Proteus mirabilis uridine + phosphate
-
?
3.1.4.16 3'-UMP + H2O
-
Proteus vulgaris uridine + phosphate
-
?
3.1.4.16 3'-UMP + H2O
-
Vibrio alginolyticus uridine + phosphate
-
?
3.1.4.16 3'-UMP + H2O
-
Shigella sonnei uridine + phosphate
-
?
3.1.4.16 3'-UMP + H2O
-
Escherichia coli B / ATCC 11303 uridine + phosphate
-
?
3.1.4.16 cyclic 2',3'-AMP + H2O
-
Escherichia coli 3'-AMP
-
?
3.1.4.16 cyclic 2',3'-AMP + H2O
-
Proteus vulgaris 3'-AMP
-
?
3.1.4.16 cyclic 2',3'-AMP + H2O
-
Shigella sonnei 3'-AMP
-
?
3.1.4.16 cyclic 2',3'-CMP + H2O
-
Escherichia coli 3'-CMP
-
?
3.1.4.16 cyclic 2',3'-CMP + H2O
-
Proteus mirabilis 3'-CMP
-
?
3.1.4.16 cyclic 2',3'-CMP + H2O
-
Proteus vulgaris 3'-CMP
-
?
3.1.4.16 cyclic 2',3'-CMP + H2O
-
Vibrio alginolyticus 3'-CMP
-
?
3.1.4.16 cyclic 2',3'-CMP + H2O
-
Shigella sonnei 3'-CMP
-
?
3.1.4.16 cyclic 2',3'-GMP + H2O
-
Escherichia coli 3'-GMP
-
?
3.1.4.16 cyclic 2',3'-GMP + H2O
-
Proteus mirabilis 3'-GMP
-
?
3.1.4.16 cyclic 2',3'-GMP + H2O
-
Vibrio alginolyticus 3'-GMP
-
?
3.1.4.16 cyclic 2',3'-UMP + H2O
-
Escherichia coli 3'-UMP
-
?
3.1.4.16 cyclic 2',3'-UMP + H2O
-
Proteus mirabilis 3'-UMP
-
?
3.1.4.16 cyclic 2',3'-UMP + H2O
-
Proteus vulgaris 3'-UMP
-
?
3.1.4.16 cyclic 2',3'-UMP + H2O
-
Vibrio alginolyticus 3'-UMP
-
?
3.1.4.16 cyclic 2',3'-UMP + H2O
-
Shigella sonnei 3'-UMP
-
?
3.1.4.16 di-p-nitrophenyl phosphate + H2O
-
Escherichia coli p-nitrophenol + p-nitrophenyl phosphate
-
?
3.1.4.16 di-p-nitrophenyl phosphate + H2O
-
Proteus mirabilis p-nitrophenol + p-nitrophenyl phosphate
-
?
3.1.4.16 di-p-nitrophenyl phosphate + H2O
-
Proteus vulgaris p-nitrophenol + p-nitrophenyl phosphate
-
?
3.1.4.16 di-p-nitrophenyl phosphate + H2O
-
Vibrio alginolyticus p-nitrophenol + p-nitrophenyl phosphate
-
?
3.1.4.16 di-p-nitrophenyl phosphate + H2O
-
Shigella sonnei p-nitrophenol + p-nitrophenyl phosphate
-
?
3.1.4.16 additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Bacillus subtilis ?
-
?
3.1.4.16 additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Escherichia coli ?
-
?
3.1.4.16 additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Serratia marcescens ?
-
?
3.1.4.16 additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Proteus mirabilis ?
-
?
3.1.4.16 additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Proteus vulgaris ?
-
?
3.1.4.16 additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Vibrio alginolyticus ?
-
?
3.1.4.16 additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Salmonella enterica subsp. enterica serovar Heidelberg ?
-
?
3.1.4.16 additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Shigella sonnei ?
-
?
3.1.4.16 additional information 3'-nucleotidase and 5'-nucleotidase activity resides in the same protein Escherichia coli B / ATCC 11303 ?
-
?
3.1.4.16 p-nitrophenyl phosphate + H2O
-
Proteus vulgaris p-nitrophenol + phosphate
-
?
3.1.4.16 p-nitrophenyl phosphate + H2O
-
Shigella sonnei p-nitrophenol + phosphate
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.16 55
-
partial inactivation Proteus mirabilis
3.1.4.16 55
-
partial inactivation Enterobacter sp.
3.1.4.16 55
-
partial inactivation Vibrio alginolyticus
3.1.4.16 55
-
5 min, activates Escherichia coli
3.1.4.16 65
-
partial inactivation Proteus mirabilis
3.1.4.16 70
-
5 min, stable Bacillus subtilis
3.1.4.16 70
-
5 min, loss of activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.16 6.4
-
-
Escherichia coli
3.1.4.16 6.5
-
-
Bacillus subtilis
3.1.4.16 7.2 8
-
Enterobacter sp.
3.1.4.16 7.5 8
-
Escherichia coli
3.1.4.16 7.6 8.3
-
Escherichia coli