Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fensome, A.C.; Rodrigues-Lima, F.; Josephs, M.; Paterson, H.F.; Katan, M.
    A neutral magnesium-dependent sphingomyelinase isoform associated with intracellular membranes and reversibly inhibited by reactive oxygen species (2000), J. Biol. Chem., 275, 1128-1136.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.12 2-mercaptoethanol
-
 Mus musculus
3.1.4.12 dithiothreitol activation in a dose dependent manner Mus musculus
3.1.4.12 reduced glutathione activation in a dose dependent manner Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.12 Ca2+
-
 Mus musculus
3.1.4.12 glutathione then oxidized Mus musculus
3.1.4.12 Ni2+
-
 Mus musculus
3.1.4.12 Zn2+
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.12 0.00059
-
 sphingomyelin
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 endoplasmic reticulum
-
 Mus musculus 5783
-
3.1.4.12 membrane
-
 Mus musculus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Ca2+
-
 Mus musculus
3.1.4.12 Mg2+ dependent Mus musculus
3.1.4.12 Mn2+
-
 Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.12 40000
-
 2 * 40000, SDS-PAGE and Western blotting analysis Mus musculus
3.1.4.12 90000 100000 gel filtration Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 10fold Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.12 brain
-
 Mus musculus
-
3.1.4.12 liver
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 sphingomyelin + H2O
-
 Mus musculus N-acylsphingosine + choline phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.12 dimer 2 * 40000, SDS-PAGE and Western blotting analysis Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.12 5
-
 acidic sphingomyelinase Mus musculus
3.1.4.12 7
-
 neutral sphingomyelinase Mus musculus