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Literature summary extracted from

  • Liu, B.; Hassler, D.F.; Smith, G.K.; Weaver, K.; Hannun, Y.A.
    Purification and characterization of a membrane bound neutral pH optimum magnesium-dependent and phosphatidylserine-stimulated sphingomyelinase from rat brain (1998), J. Biol. Chem., 273, 34472-34479.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.12 arachidonic acid
-
 Rattus norvegicus
3.1.4.12 dithiothreitol 2fold enhanced activity at 3 mM Rattus norvegicus
3.1.4.12 lyso-phosphatidylcholine
-
 Rattus norvegicus
3.1.4.12 phosphatidic acid
-
 Rattus norvegicus
3.1.4.12 phosphatidylethanolamine
-
 Rattus norvegicus
3.1.4.12 phosphatidylinositol
-
 Rattus norvegicus
3.1.4.12 phosphatidylserine
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.12 Cu2+
-
 Rattus norvegicus
3.1.4.12 deoxycholate
-
 Rattus norvegicus
3.1.4.12 glutathione
-
 Rattus norvegicus
3.1.4.12 Mg2+ slight inhibitory at higher concentrations Rattus norvegicus
3.1.4.12 Ni2+
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.12 additional information
-
 sphingomyelin
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Mg2+ dependent Rattus norvegicus
3.1.4.12 Mn2+
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.12 150000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 partial, 3030fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.12 brain
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
3.1.4.12 4°C, 14 days, Triton X-100 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 sphingomyelin + H2O
-
 Rattus norvegicus N-acylsphingosine + choline phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.12 ? x * 55000-90000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.12 7
-
 neutral sphingomyelinase Rattus norvegicus
3.1.4.12 7.5
-
-
 Rattus norvegicus