BRENDA - Enzyme Database

Characteristics and partial purification of a novel cytosolic, magnesium-independent, neutral sphingomyelinase activated in the early signal transduction of 1alpha,25-dihydroxyvitamin D3-induced HL-60 cell differentiation

Okazaki, T.; Bielawska, A.; Domae, N.; Bell, R.M.; Hannun, Y.A.; J. Biol. Chem. 269, 4070-4077 (1994)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.12
deoxycholate
-
Homo sapiens
3.1.4.12
additional information
-
Homo sapiens
3.1.4.12
phosphatidic acid
-
Homo sapiens
3.1.4.12
phosphatidylinositol
-
Homo sapiens
3.1.4.12
phosphatidylserine
-
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.12
Ca2+
slight
Homo sapiens
3.1.4.12
Cu2+
-
Homo sapiens
3.1.4.12
Fe3+
-
Homo sapiens
3.1.4.12
additional information
-
Homo sapiens
3.1.4.12
Zn2+
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.12
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.12
45000
-
1 * 95000 + 1 * 45000, gel filtration
Homo sapiens
3.1.4.12
95000
-
1 * 95000 + 1 * 45000, gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.12
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.12
4475fold
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.12
HL-60 cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.12
additional information
-
different assay conditions, substrates etc.
Homo sapiens
Subunits
EC Number
Subunits
Commentary
Organism
3.1.4.12
dimer
1 * 95000 + 1 * 45000, gel filtration
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.12
7.5
-
-
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.12
deoxycholate
-
Homo sapiens
3.1.4.12
additional information
-
Homo sapiens
3.1.4.12
phosphatidic acid
-
Homo sapiens
3.1.4.12
phosphatidylinositol
-
Homo sapiens
3.1.4.12
phosphatidylserine
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.12
Ca2+
slight
Homo sapiens
3.1.4.12
Cu2+
-
Homo sapiens
3.1.4.12
Fe3+
-
Homo sapiens
3.1.4.12
additional information
-
Homo sapiens
3.1.4.12
Zn2+
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.12
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.12
45000
-
1 * 95000 + 1 * 45000, gel filtration
Homo sapiens
3.1.4.12
95000
-
1 * 95000 + 1 * 45000, gel filtration
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.12
4475fold
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.12
HL-60 cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.12
additional information
-
different assay conditions, substrates etc.
Homo sapiens
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.4.12
dimer
1 * 95000 + 1 * 45000, gel filtration
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.12
7.5
-
-
Homo sapiens