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Literature summary extracted from

  • Blank, J.L.; Foster, K.A.; Hawthorne, J.N.
    Purification, properties, and phosphorylation by protein kinase C of two phosphoinositidase C isoenzymes from rat brain (1991), J. Neurochem., 57, 15-21.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.11 albumin maximal activation of hydrolysis of phosphatidylinositol 4,5-diphosphate at 0.0125 mg/ml Rattus norvegicus
3.1.4.11 cytochrome c maximal activation of hydrolysis of phosphatidylinositol 4,5-diphosphate at 0.0125 mg/ml Rattus norvegicus
3.1.4.11 histone maximal activation of hydrolysis of phosphatidylinositol 4,5-diphosphate at 0.0125 mg/ml Rattus norvegicus
3.1.4.11 additional information rat brain protein kinase C phosphorylates phosphoinositidase gamma but not phosphoinositidase beta1. Incubation with the kinase increases the activity of both enzymes however, possibly by phosphorylation of another protein in the preparations Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.11 0.012
-
 phosphatidylinositol isoenzyme PLC-gamma Rattus norvegicus
3.1.4.11 0.031
-
 phosphatidylinositol isoenzyme PLC-gamma Rattus norvegicus
3.1.4.11 0.035
-
 phosphatidylinositol isoenzyme PLC-beta1 Rattus norvegicus
3.1.4.11 0.058
-
 phosphatidylinositol 4,5-bisphosphate isoenzyme PLC-beta1 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.11 Ca2+ absolute requirement for both isoenzymes. Maximal activity with phosphatidylinositol at pH 5.5: 1 mM. Maximal activity with phosphatidylinositol 4,5-diphosphate at pH 7.0: 0.1 mM Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.11 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.4.11 side-chain modification rat brain protein kinase C phosphorylates phosphoinositidase gamma but not phosphoinositidase beta1 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.11
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.11 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.11 3.1
-
 isoenzyme PLC-gamma Rattus norvegicus
3.1.4.11 11.4
-
 isoenzyme PLC-beta1 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.11 phosphatidylinositol + H2O phosphatidylinositol 4,5-bisphosphate is a better substrate Rattus norvegicus ?
-
 ?
3.1.4.11 phosphatidylinositol 4,5-bisphosphate + H2O
-
 Rattus norvegicus D-myo-inositol 1,4,5-trisphosphate + diacylglycerol isoenzyme PLC beta1 produces 21% inositol 1:2,4,5 cyclic trisphosphate at pH 5.5 and 7.5% at pH 7.0 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.11 5 5.5 hydrolysis of phosphatidylinositol, isoenzyme PLC-beta-1 and PLC-gamma, in presence of 1 mM CaCl2 Rattus norvegicus
3.1.4.11 6.5 7 hydrolysis of phosphatidylinositol, isoenzyme PLC-beta1 in presence of 0.6 mg/ml sodium deoxycholate, isoenzyme PLC-gamma in presence of 0.4 mg/ml sodium deoxycholate Rattus norvegicus
3.1.4.11 7.5
-
 hydrolysis of phosphatidylinositol 4,5-diphosphate, in presence of 0.1 mM CaCl2 Rattus norvegicus