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Literature summary extracted from

  • Alexander, M.; Heppel, L.A.; Hurwitz, J.
    The purification and properties of micrococcal nuclease (1961), J. Biol. Chem., 236, 3014-3019.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.31.1 extracellular
-
 Staphylococcus aureus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.31.1 Ca2+ required Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.31.1 12000
-
 sucrose density gradient centrifugation Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.1.31.1 Staphylococcus aureus
-
 var.aureus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.31.1
-
 Staphylococcus aureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.31.1 additional information
-
-
 Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.31.1 DNA + H2O
-
 Staphylococcus aureus 3'-deoxymononucleotides + dinucleotides + oligonucleotides terminated by 3'-phosphate, produced only in incomplete digestion ?
3.1.31.1 additional information inhibition when a 5'-phosphomonoester end group is present in an oligonucleotide Staphylococcus aureus ?
-
 ?
3.1.31.1 additional information best substrates oligonucleotides with a 3'-phosphomonoester end group Staphylococcus aureus ?
-
 ?
3.1.31.1 additional information enzyme does not cleave the 2',3'-cyclic phosphate derivates of the ribonucleosides Staphylococcus aureus ?
-
 ?
3.1.31.1 RNA + H2O
-
 Staphylococcus aureus nucleoside 3'-phosphates + dinucleotides dinucleotides terminated by 3'-phosphates ?
3.1.31.1 T2 DNA + H2O
-
 Staphylococcus aureus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.31.1 additional information
-
-
 Staphylococcus aureus
3.1.31.1 89
-
 10 min Staphylococcus aureus
3.1.31.1 90
-
 pH 7.9-8.8, 15 min, 20% loss of activity, pH 3.5 and 9.6, 15 min, 50% loss of activity Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.31.1 additional information
-
-
 Staphylococcus aureus
3.1.31.1 8.6
-
-
 Staphylococcus aureus
3.1.31.1 8.8
-
-
 Staphylococcus aureus