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Literature summary extracted from
- An extracellular nuclease from suspension cultures of tobacco (1974), Biochim. Biophys. Acta, 366, 89-100.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.2 | EDTA | - |
Nicotiana tabacum |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.30.2 | extracellular | - |
Nicotiana tabacum | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.2 | additional information | no requirement for added divalent cation | Nicotiana tabacum |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.2 | 35000 | - |
gel filtration | Nicotiana tabacum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.30.2 | Nicotiana tabacum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.30.2 | - |
Nicotiana tabacum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.30.2 | culture medium | - |
Nicotiana tabacum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.2 | 3'-nucleotides + H2O | e.g. 3'-AMP | Nicotiana tabacum | ? | - |
? | |
| 3.1.30.2 | DNA + H2O | native double-stranded | Nicotiana tabacum | 5'-phosphonucleotides + 5'-phosphomononucleotides | - |
? | |
| 3.1.30.2 | DNA + H2O | single-stranded DNA | Nicotiana tabacum | 5'-phosphonucleotides + 5'-phosphomononucleotides | - |
? | |
| 3.1.30.2 | RNA + H2O | rRNA | Nicotiana tabacum | 5'-phosphooligonucleotides + 5'-phosphomononucleotides | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.2 | 5.2 | - |
native DNA | Nicotiana tabacum |
| 3.1.30.2 | 5.7 | - |
denatured DNA | Nicotiana tabacum |
| 3.1.30.2 | 6 | - |
RNA | Nicotiana tabacum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.2 | 4.2 | 9 | adenosine 3'-monophosphate, less than 20% of maximal activity above and below | Nicotiana tabacum |
| 3.1.30.2 | 4.5 | 5.7 | native DNA, less than 25% of maximal activity above and below | Nicotiana tabacum |
| 3.1.30.2 | 5 | 7 | RNA less than 10% of maximal activity above and below | Nicotiana tabacum |
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Release 2023.1 (January 2023)
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