Literature summary extracted from
Stevens, A.; Hilmoe, R.J.
Studies on a nuclease from Azotobacter agilis. II. Hydrolysis of ribonucleic and deoxyribonucleic acids (1960), J. Biol. Chem., 235, 3023-3027.
No PubMed abstract available
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.1.30.2 |
Urea |
2 M, stimulation |
Azotobacter agilis |
|
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.30.2 |
Mg2+ |
increases hydrolysis rate of EDTA-dialyzed RNA, no effect on hydrolysis of undialyzed RNA, inhibition at high concentrations |
Azotobacter agilis |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.30.2 |
Mg2+ |
increases hydrolysis rate of EDTA-dialyzed RNA, no effect on hydrolysis of undialyzed RNA, inhibition at high concentrations |
Azotobacter agilis |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.30.2 |
Azotobacter agilis |
- |
similar enzyme |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.30.2 |
DNA + H2O |
- |
Azotobacter agilis |
5'-phosphonucleotides + 5'-phosphomononucleotides |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.30.2 |
More |
similar enzymes: silkworm nuclease, potato nuclease, Azotobacter nuclease |
Azotobacter agilis |
3.1.30.2 |
More |
mung bean nuclease has specificity for single-stranded substrates, see E.C. 3.1.30.1 |
Azotobacter agilis |