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Literature summary extracted from

  • Stevens, A.; Hilmoe, R.J.
    Studies on a nuclease from Azotobacter agilis. I. Isolation and mode of action (1960), J. Biol. Chem., 235, 3016-3022.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.30.2 additional information secondary interaction in long RNA chains inhibit the enzyme Azotobacter agilis

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.2 Azotobacter agilis
-
 similar enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.30.2 similar enzyme Azotobacter agilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.30.2 additional information
-
-
 Azotobacter agilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.2 DNA + H2O
-
 Azotobacter agilis 5'-phosphonucleotides + 5'-phosphomononucleotides
-
 ?
3.1.30.2 additional information hydrolysis of shorter chains much slower than of longer chains Azotobacter agilis ?
-
 ?
3.1.30.2 RNA + H2O polyuridylic acid Azotobacter agilis 5'-phosphooligonucleotides + 5'-phosphomononucleotides
-
 ?
3.1.30.2 RNA + H2O polycytidylic acid Azotobacter agilis 5'-phosphooligonucleotides + 5'-phosphomononucleotides
-
 ?
3.1.30.2 RNA + H2O e.g.: polyadenylic acid Azotobacter agilis 5'-phosphooligonucleotides + 5'-phosphomononucleotides
-
 ?
3.1.30.2 RNA + H2O not polyguanylic acid Azotobacter agilis 5'-phosphooligonucleotides + 5'-phosphomononucleotides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.30.2 More similar enzymes: silkworm nuclease, potato nuclease, Azotobacter nuclease Azotobacter agilis
3.1.30.2 More mung bean nuclease has specificity for single-stranded substrates, see E.C. 3.1.30.1 Azotobacter agilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.30.2 7.7
-
-
 Azotobacter agilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.30.2 7.7 8.5 sharp decrease in activity below and above Azotobacter agilis