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Literature summary extracted from

  • Ikeda, S.; Maeda, N.; Ohshima, T.; Takata, N.
    Identification and characterization of a mitochondrial endonuclease from yeast, Schizosaccharomyces pombe (1996), Biochem. Mol. Biol. Int., 40, 1017-1024.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.30.1 aurintricarboxylic acid complete inhibition at 0.005 mM Schizosaccharomyces pombe
3.1.30.1 Ca2+ about 10% of activity towards ssDNA at 1 and 10 mM Schizosaccharomyces pombe
3.1.30.1 Ionic strength about 40% of activity towards ssDNA at 0.2 M NaCl and KCl Schizosaccharomyces pombe
3.1.30.1 Mg2+ reduction of activity towards ssDNA at 10 mM Schizosaccharomyces pombe
3.1.30.1 Zn2+ about 15% of activity towards ssDNA at 1 and 10 mM Schizosaccharomyces pombe

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.30.1 mitochondrion
-
 Schizosaccharomyces pombe 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.30.1 Mg2+
-
 Schizosaccharomyces pombe
3.1.30.1 Mn2+ required for activity, reduced activity towards ssDNA at 10 mM Schizosaccharomyces pombe

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.30.1 32000
-
 SDS-PAGE Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.1 DNA + H2O Schizosaccharomyces pombe
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 RNA + H2O Schizosaccharomyces pombe
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.1 Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.30.1
-
 Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.1 DNA + H2O
-
 Schizosaccharomyces pombe 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 dsDNA + H2O
-
 Schizosaccharomyces pombe 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 RNA + H2O
-
 Schizosaccharomyces pombe 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 ssDNA + H2O
-
 Schizosaccharomyces pombe 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.30.1 Schizosaccharomyces pombe endonuclease
-
 Schizosaccharomyces pombe

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.30.1 37
-
-
 Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.30.1 7.5
-
-
 Schizosaccharomyces pombe