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Literature summary extracted from
- Partial purification and properties of an endonuclease from germinating pea seeds specific for single-stranded DNA (1979), Arch. Biochem. Biophys., 196, 138-146.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.1 | 8-hydroxychinoline 5-sulfonate | with 1 mM 50% of maximal activity on denaturated DNA, 70% with native DNA | Pisum sativum |  |
| 3.1.30.1 | Hg2+ | complete inactivation by 5 mM HgCl2 | Pisum sativum | |
| 3.1.30.1 | NaCl | inhibition at concentrations higher than 100 mM | Pisum sativum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.1 | Ca2+ | 60% stimulation of activity on denaturated DNA by 5 mM CaCl2 | Pisum sativum | |
| 3.1.30.1 | Mg2+ | 40% stimulation of activity on denaturated DNA by 5 mM MgCl2 | Pisum sativum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 42000 | - |
gel filtration | Pisum sativum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.1 | DNA + H2O | Pisum sativum | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.30.1 | Pisum sativum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.30.1 | - |
Pisum sativum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.30.1 | seed | germinating | Pisum sativum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.1 | DNA + H2O | - |
Pisum sativum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | dsDNA + H2O | 15-20fold higher activity on single-stranded than native DNA | Pisum sativum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Pisum sativum | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 45 | - |
with denaturated DNA as substrate | Pisum sativum |
| 3.1.30.1 | 60 | - |
with native DNA or 3'-AMP as substrate | Pisum sativum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 20 | 80 | - |
Pisum sativum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 7.5 | - |
in Tris-HCl-buffer, at this pH only 30% of this activity in potassium phosphate buffer, only 60% in sodium citrate buffer | Pisum sativum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 6.5 | 8 | - |
Pisum sativum |
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Release 2023.1 (January 2023)
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