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Literature summary extracted from

  • Tomkinson, A.E.; Linn, S.
    Purification and properties of a single strand-specific endonuclease from mouse cell mitochondria (1986), Nucleic Acids Res., 14, 9579-9583.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.30.1 EDTA almost no activity with 2 mM Mus musculus
3.1.30.1 KCl 50% of activity with 0.019 and 0.025 mM Mus musculus
3.1.30.1 NaCl inhibition by low concentrations, 50% of activity with 19 and 0.025 mM Mus musculus
3.1.30.1 phosphate 45% of maximal activity with 20 mM potassium phosphate, 26% with 30 mM Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.30.1 mitochondrion
-
 Mus musculus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.30.1 Co2+ optimal concentration 2-10 mM CoCl2 Mus musculus
3.1.30.1 Mg2+ optimal concentration 1-2 mM MgCl2 Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.30.1 37400
-
 SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.1 DNA + H2O Mus musculus
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 RNA + H2O Mus musculus
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.1 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.30.1 from mitochondria Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.30.1 MPC-11 cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.30.1 7.7
-
 mitochondrial activity with ssDNA as substrate Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.1 DNA + H2O
-
 Mus musculus 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 dsDNA + H2O
-
 Mus musculus 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 RNA + H2O
-
 Mus musculus 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 ssDNA + H2O heat denaturated, 100°C, 5-15 min Mus musculus 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.30.1 7.5
-
-
 Mus musculus