Any feedback?
Literature summary extracted from
- An endonuclease from mouse cells specific for single-stranded DNA (1976), Eur. J. Biochem., 71, 617-622.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.1 | dimethyl sulfoxide | - |
Mus musculus |  |
| 3.1.30.1 | glycerol | 2fold stimulation of activity by 10-20% in 50 mM Tris-HCl-buffer, pH 7.5 | Mus musculus | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.1 | Ca2+ | 30% of activity towards denaturated ssDNA with 1.2 mM | Mus musculus | |
| 3.1.30.1 | Mg2+ | concentrations higher 2 mM | Mus musculus | |
| 3.1.30.1 | NaCl | complete inhibition with 50 mM | Mus musculus | |
| 3.1.30.1 | sodium dodecylsulfate | complete inhibition with 0.2% | Mus musculus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.1 | Mg2+ | optimal concentration 1-2 mM MgCl2 | Mus musculus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 76000 | - |
- |
Mus musculus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.1 | DNA + H2O | Mus musculus | - |
5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.30.1 | Mus musculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.30.1 | - |
Mus musculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.30.1 | ascites tumor cell | - |
Mus musculus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.1 | DNA + H2O | - |
Mus musculus | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
| 3.1.30.1 | ssDNA + H2O | - |
Mus musculus | 5'-phosphomononucleotides + 5'-phosphooligonucleotides | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 7.5 | - |
with 10 mM sodium phosphate buffer | Mus musculus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 6.5 | 8.5 | - |
Mus musculus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
