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Literature summary extracted from

  • Gite, S.; Shankar, V.
    Preparation, properties and application of Aspergillus oryzae S1 nuclease covalently bound to aminobutyl-Bio-Gel P-2 through its carbohydrate moiety (1993), Biotechnol. Appl. Biochem., 17, 373-382.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.1 DNA + H2O Aspergillus oryzae
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.1 Aspergillus oryzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.30.1
-
 Aspergillus oryzae

Storage Stability

EC Number Storage Stability Organism
3.1.30.1 4°C, 30 mM sodium acetate buffer pH 4.6, containing 1 mM ZnSO4, 50 mM NaCl and 5% glycerol, more than 45 days, no apparent loss of activity Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.1 DNA + H2O
-
 Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 ssDNA + H2O
-
 Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.30.1 S1 nuclease
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.30.1 37
-
-
 Aspergillus oryzae
3.1.30.1 55
-
 soluble and immobilized enzyme Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.30.1 30 70 at 70°C almost 75% of initial activity with immobilized, 20% with soluble enzyme Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.30.1 4.6
-
-
 Aspergillus oryzae