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Literature summary extracted from

  • Gite, S.; Shankar, V.
    Characterization of S1 nuclease. Involvement of carboxylate groups in metal binding (1992), Eur. J. Biochem., 210, 437-441.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.30.1 carboxylate group modification at pH 4.6, 50-85% loss of initial activity towards ssDNA, RNA and 3'-AMP after modification of carboxylate groups with 5-15 mM 1-ethyl-3-(dimethylaminopropyl)-carbodiimide; at pH 7.8, 55-95% loss of initial activity towards ssDNA, RNA and 3'-AMP after modification of carboxylate groups with 2-15 mM Woodward's reagent K Aspergillus oryzae
3.1.30.1 EDTA 55-100% loss of initial activity with 1-10 mM Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.30.1 Co2+ with 1 mM restoration of 72% of activity of 1 mM EDTA-treated enzyme Aspergillus oryzae
3.1.30.1 Zn2+ with 1 mM restoration of 76% of activity of 1 mM EDTA-treated enzyme Aspergillus oryzae
3.1.30.1 Zn2+ metalloprotein with one loosely and two strongly bound zinc atoms Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.1 DNA + H2O Aspergillus oryzae
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 RNA + H2O Aspergillus oryzae
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.1 Aspergillus oryzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.30.1
-
 Aspergillus oryzae

Storage Stability

EC Number Storage Stability Organism
3.1.30.1 -20°C, purified enzyme after dialysis against deionized water, no loss of activity Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.1 3'-AMP + H2O
-
 Aspergillus oryzae adenosine + phosphate
-
 ?
3.1.30.1 DNA + H2O
-
 Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 RNA + H2O
-
 Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 ssDNA + H2O
-
 Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.30.1 S1 nuclease
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.30.1 37
-
-
 Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.30.1 4.6
-
-
 Aspergillus oryzae