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Literature summary extracted from

  • Vogt, V.M.
    Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae (1973), Eur. J. Biochem., 33, 192-200.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.30.1 NaCl optimal activity at 100 mM, 55% of maximal rate at 400 mM Aspergillus oryzae
3.1.30.1 sodium dodecylsulfate above 0.04% decrease of reaction rate. 60% of activity at 0.12% Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.30.1 Cd2+ only marginal stimulating effects Aspergillus oryzae
3.1.30.1 Co2+
-
 Aspergillus oryzae
3.1.30.1 Mn2+ only marginal stimulating effects Aspergillus oryzae
3.1.30.1 Zn2+
-
 Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.30.1 32000
-
 SDS-PAGE Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.1 DNA + H2O Aspergillus oryzae
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 RNA + H2O Aspergillus oryzae
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.1 Aspergillus oryzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.30.1
-
 Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.1 DNA + H2O
-
 Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 polyadenylic acid + H2O
-
 Aspergillus oryzae 5'-AMP + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 polycytosylic acid + H2O
-
 Aspergillus oryzae 5'-CMP + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 polyguanylic acid + H2O
-
 Aspergillus oryzae 5'-GMP + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 polyuridylic acid + H2O
-
 Aspergillus oryzae 5'-UMP + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 RNA + H2O
-
 Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 ssDNA + H2O heat denaturated, 100°C, 5-15 min Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.30.1 single-strand-specific nuclease
-
 Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.30.1 4 4.3
-
 Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.30.1 3.1 6.4 almost 50% of activity at pH 3.3 and 4.6 Aspergillus oryzae