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Literature summary extracted from

  • Ando, T.
    A nuclease specific for heat denaturated DNA isolated from a product of Aspergillus oryzae (1966), Biochim. Biophys. Acta, 114, 158-168.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.30.1 EDTA 69% of maximum activity at 20 mM Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.30.1 Zn2+ 36% activation at 0.1 mM, 16% activation at 1 mM Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.1 DNA + H2O Aspergillus oryzae
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.1 Aspergillus oryzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.30.1
-
 Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.1 bis(p-nitrophenyl)phosphate + H2O almost no hydrolysis Aspergillus oryzae p-nitrophenol + phosphate
-
 ?
3.1.30.1 DNA + H2O
-
 Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 ssDNA + H2O heat denaturated, 100°C, 5-15 min Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.30.1 4.4 4.6
-
 Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.30.1 3 8 almost 50% of activity at pH 3.0 and 5.5 Aspergillus oryzae