BRENDA - Enzyme Database

Characterization of dolichol and dolichyl phosphate phosphatase from soya beans (Glycine max)

Ravi, K.; Rip, J.W.; Carroll, K.K.; Biochem. J. 213, 513-518 (1983)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.51
EDTA
enhances activity
Glycine max
3.1.3.51
phosphatidylcholine
0.1 mM, slight stimulation
Glycine max
3.1.3.51
phosphatidylethanolamine
0.1 mM, slight stimulation
Glycine max
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.51
lysophosphatidic acid
0.1 mM or 1 mM
Glycine max
3.1.3.51
phosphatidic acid
0.1 mM or 1 mM, competitive
Glycine max
3.1.3.51
phosphatidylcholine
1 mM
Glycine max
3.1.3.51
phosphatidylethanolamine
1 mM
Glycine max
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.51
0.005
-
dolichyl phosphate
-
Glycine max
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.51
microsome
-
Glycine max
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.51
Glycine max
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.51
cotyledon
-
Glycine max
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.51
dolichyl phosphate + H2O
-
134920
Glycine max
dolichol + phosphate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.51
5
10
pH 5: about 35% of maximal activity, pH 10.0: about 85% of maximal activity
Glycine max
3.1.3.51
7
9
-
Glycine max
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.51
EDTA
enhances activity
Glycine max
3.1.3.51
phosphatidylcholine
0.1 mM, slight stimulation
Glycine max
3.1.3.51
phosphatidylethanolamine
0.1 mM, slight stimulation
Glycine max
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.51
lysophosphatidic acid
0.1 mM or 1 mM
Glycine max
3.1.3.51
phosphatidic acid
0.1 mM or 1 mM, competitive
Glycine max
3.1.3.51
phosphatidylcholine
1 mM
Glycine max
3.1.3.51
phosphatidylethanolamine
1 mM
Glycine max
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.51
0.005
-
dolichyl phosphate
-
Glycine max
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.51
microsome
-
Glycine max
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.51
cotyledon
-
Glycine max
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.51
dolichyl phosphate + H2O
-
134920
Glycine max
dolichol + phosphate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.51
5
10
pH 5: about 35% of maximal activity, pH 10.0: about 85% of maximal activity
Glycine max
3.1.3.51
7
9
-
Glycine max