BRENDA - Enzyme Database

Dolichyl-phosphate phosphatase and dolichyl-diphosphate phosphatase in rat-liver microsomes

Belocopitow, E.; Boscoboinik, D.; Eur. J. Biochem. 125, 167-173 (1982)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.6.1.43
Triton X-100
optimum activity above 0.14%, stable up to 4.2%
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.51
Ca2+
16 mM CaCl2, 24% loss of activity
Rattus norvegicus
3.1.3.51
CoCl2
16 mM, 31% inhibition
Rattus norvegicus
3.1.3.51
diphosphate
-
Rattus norvegicus
3.1.3.51
dolichol
-
Rattus norvegicus
3.1.3.51
Mn2+
16 mM MnCl2, 35% inhibition
Rattus norvegicus
3.1.3.51
phosphate
-
Rattus norvegicus
3.1.3.51
phosphatidylcholine
-
Rattus norvegicus
3.1.3.51
phosphatidylethanolamine
-
Rattus norvegicus
3.6.1.43
divalent cations
-
Rattus norvegicus
3.6.1.43
dolichol
-
Rattus norvegicus
3.6.1.43
phosphate
slight inhibition
Rattus norvegicus
3.6.1.43
phosphatidic acid
70% inhibition at 60 mM
Rattus norvegicus
3.6.1.43
phosphatidylcholine
nearly full inhibition
Rattus norvegicus
3.6.1.43
phosphatidylethanolamine
-
Rattus norvegicus
3.6.1.43
Triton X-100
activating
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.51
0.15
-
dolichyl phosphate
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.51
microsome
-
Rattus norvegicus
-
-
3.6.1.43
microsome
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.43
additional information
inhibited by divalent cations
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.43
dolichyl diphosphate + H2O
Rattus norvegicus
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
dolichyl phosphate + phosphate
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.51
Rattus norvegicus
-
-
-
3.6.1.43
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.51
liver
-
Rattus norvegicus
-
3.6.1.43
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.51
dolichyl phosphate + H2O
-
134917
Rattus norvegicus
dolichol + phosphate
-
-
-
?
3.6.1.43
dolichyl diphosphate + H2O
-
134917
Rattus norvegicus
dolichyl phosphate + phosphate
-
134917
Rattus norvegicus
?
3.6.1.43
dolichyl diphosphate + H2O
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
134917
Rattus norvegicus
dolichyl phosphate + phosphate
-
134917
Rattus norvegicus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.6.1.43
30
-
-
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.43
6.9
-
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.6.1.43
Triton X-100
optimum activity above 0.14%, stable up to 4.2%
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.51
Ca2+
16 mM CaCl2, 24% loss of activity
Rattus norvegicus
3.1.3.51
CoCl2
16 mM, 31% inhibition
Rattus norvegicus
3.1.3.51
diphosphate
-
Rattus norvegicus
3.1.3.51
dolichol
-
Rattus norvegicus
3.1.3.51
Mn2+
16 mM MnCl2, 35% inhibition
Rattus norvegicus
3.1.3.51
phosphate
-
Rattus norvegicus
3.1.3.51
phosphatidylcholine
-
Rattus norvegicus
3.1.3.51
phosphatidylethanolamine
-
Rattus norvegicus
3.6.1.43
divalent cations
-
Rattus norvegicus
3.6.1.43
dolichol
-
Rattus norvegicus
3.6.1.43
phosphate
slight inhibition
Rattus norvegicus
3.6.1.43
phosphatidic acid
70% inhibition at 60 mM
Rattus norvegicus
3.6.1.43
phosphatidylcholine
nearly full inhibition
Rattus norvegicus
3.6.1.43
phosphatidylethanolamine
-
Rattus norvegicus
3.6.1.43
Triton X-100
activating
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.51
0.15
-
dolichyl phosphate
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.51
microsome
-
Rattus norvegicus
-
-
3.6.1.43
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.43
additional information
inhibited by divalent cations
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.43
dolichyl diphosphate + H2O
Rattus norvegicus
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
dolichyl phosphate + phosphate
-
Rattus norvegicus
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.51
liver
-
Rattus norvegicus
-
3.6.1.43
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.51
dolichyl phosphate + H2O
-
134917
Rattus norvegicus
dolichol + phosphate
-
-
-
?
3.6.1.43
dolichyl diphosphate + H2O
-
134917
Rattus norvegicus
dolichyl phosphate + phosphate
-
134917
Rattus norvegicus
?
3.6.1.43
dolichyl diphosphate + H2O
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
134917
Rattus norvegicus
dolichyl phosphate + phosphate
-
134917
Rattus norvegicus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.6.1.43
30
-
-
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.43
6.9
-
-
Rattus norvegicus