BRENDA - Enzyme Database

Dolichyl phosphate phosphatase in rat liver microsomes. Avoidance of the use of detergent in testing the effect of phospholipids on dolichyl phosphate phosphatase

Boscoboinik, D.O.; Morera, S.; Belocopitow, E.; Biochim. Biophys. Acta 794, 41-48 (1984)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.51
ADP
activates
Rattus norvegicus
3.1.3.51
AMP
activates
Rattus norvegicus
3.1.3.51
ATP
activates
Rattus norvegicus
3.1.3.51
EDTA
activates
Rattus norvegicus
3.1.3.51
glucose 1-phosphate
activates
Rattus norvegicus
3.1.3.51
glucose 6-phosphate
activates
Rattus norvegicus
3.1.3.51
phosphatidylcholine
activates
Rattus norvegicus
3.1.3.51
phosphatidylethanolamine
activates
Rattus norvegicus
3.1.3.51
sphingomyelin
activates
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.51
NaF
slight inhibition
Rattus norvegicus
3.1.3.51
phosphate
-
Rattus norvegicus
3.1.3.51
phosphatidic acid
0.00006 mg, 50% inhibition
Rattus norvegicus
3.1.3.51
phosphatidylinositol
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.51
microsome
-
Rattus norvegicus
-
-
3.1.3.51
plasma membrane
-
Rattus norvegicus
5886
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.51
300000
-
gel filtration, sucrose density gradient centrifugation
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.51
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.51
liver
-
Rattus norvegicus
-
Storage Stability
EC Number
Storage Stability
Organism
3.1.3.51
-20°C, 24 h, 35% loss of activity
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.51
dolichyl phosphate + H2O
-
134915
Rattus norvegicus
dolichol + phosphate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.51
30
-
dolichyl phosphate solution
Rattus norvegicus
3.1.3.51
37
-
dolichyl phosphate particles
Rattus norvegicus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.3.51
0.00006
-
phosphatidic acid
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.51
ADP
activates
Rattus norvegicus
3.1.3.51
AMP
activates
Rattus norvegicus
3.1.3.51
ATP
activates
Rattus norvegicus
3.1.3.51
EDTA
activates
Rattus norvegicus
3.1.3.51
glucose 1-phosphate
activates
Rattus norvegicus
3.1.3.51
glucose 6-phosphate
activates
Rattus norvegicus
3.1.3.51
phosphatidylcholine
activates
Rattus norvegicus
3.1.3.51
phosphatidylethanolamine
activates
Rattus norvegicus
3.1.3.51
sphingomyelin
activates
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.51
NaF
slight inhibition
Rattus norvegicus
3.1.3.51
phosphate
-
Rattus norvegicus
3.1.3.51
phosphatidic acid
0.00006 mg, 50% inhibition
Rattus norvegicus
3.1.3.51
phosphatidylinositol
-
Rattus norvegicus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.3.51
0.00006
-
phosphatidic acid
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.51
microsome
-
Rattus norvegicus
-
-
3.1.3.51
plasma membrane
-
Rattus norvegicus
5886
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.51
300000
-
gel filtration, sucrose density gradient centrifugation
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.51
liver
-
Rattus norvegicus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.3.51
-20°C, 24 h, 35% loss of activity
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.51
dolichyl phosphate + H2O
-
134915
Rattus norvegicus
dolichol + phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.51
30
-
dolichyl phosphate solution
Rattus norvegicus
3.1.3.51
37
-
dolichyl phosphate particles
Rattus norvegicus