BRENDA - Enzyme Database

Enzymatic dephosphorylation of endogenous and exogenous dolichyl monophosphate by calf brain membranes

Burton, W.A.; Scher, M.G.; Waechter, C.J.; Arch. Biochem. Biophys. 208, 409-417 (1981)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.51
Ca2+
CaCl2
Bos taurus
3.1.3.51
MgCl2
weak inhibition
Bos taurus
3.1.3.51
Mn2+
10 mM, 57% inhibition
Bos taurus
3.1.3.51
NaF
10 mM, 82% loss of activity
Bos taurus
3.1.3.51
retinyl monophosphate
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.51
0.2
-
dolichyl phosphate
-
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.51
plasma membrane
-
Bos taurus
5886
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.51
Bos taurus
-
calf
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.51
brain
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.51
dolichyl phosphate + H2O
-
134914
Bos taurus
dolichol + phosphate
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.51
60
-
3 min, 2% loss of activity
Bos taurus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.51
5
9
pH 5: about 35% of maximal activity, pH 9: about 50% of maximal activity
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.51
Ca2+
CaCl2
Bos taurus
3.1.3.51
MgCl2
weak inhibition
Bos taurus
3.1.3.51
Mn2+
10 mM, 57% inhibition
Bos taurus
3.1.3.51
NaF
10 mM, 82% loss of activity
Bos taurus
3.1.3.51
retinyl monophosphate
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.51
0.2
-
dolichyl phosphate
-
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.51
plasma membrane
-
Bos taurus
5886
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.51
brain
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.51
dolichyl phosphate + H2O
-
134914
Bos taurus
dolichol + phosphate
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.51
60
-
3 min, 2% loss of activity
Bos taurus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.51
5
9
pH 5: about 35% of maximal activity, pH 9: about 50% of maximal activity
Bos taurus