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Literature summary extracted from

  • Adrian, G.S.; Keenan, R.W.
    A dolichyl phosphate-cleaving acid phosphatase from Tetrahymena pyriformis (1979), Biochim. Biophys. Acta, 575, 431-438.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.51 ethoxy formic anhydride 10 mM, 67% inhibition of dolichyl phosphate hydrolysis, no inhibition of p-nitrophenyl phosphate cleavage Tetrahymena pyriformis
3.1.3.51 Tartrate inhibits hydrolysis of p-nitrophenyl phosphate, no inhibition of the hydrolysis of dolichyl phosphate Tetrahymena pyriformis
3.1.3.51 vanadium oxide inhibits hydrolysis of p-nitrophenyl phosphate and dolichyl phosphate Tetrahymena pyriformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.51 Mg2+ hydrolysis of dolichyl phosphate requires Mg2+ Tetrahymena pyriformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.51 additional information Tetrahymena pyriformis the enzyme is required for the regulation of the level of dolichyl phosphate and thus the rate of glycoprotein synthesis ?
-
 ?
3.1.3.51 additional information Tetrahymena pyriformis WH14 the enzyme is required for the regulation of the level of dolichyl phosphate and thus the rate of glycoprotein synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.51 Tetrahymena pyriformis
-
-
-
3.1.3.51 Tetrahymena pyriformis WH14
-
-
-

Storage Stability

EC Number Storage Stability Organism
3.1.3.51 -20°C, stable for several months Tetrahymena pyriformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.51 dolichyl phosphate + H2O
-
 Tetrahymena pyriformis dolichol + phosphate
-
 ?
3.1.3.51 dolichyl phosphate + H2O
-
 Tetrahymena pyriformis WH14 dolichol + phosphate
-
 ?
3.1.3.51 additional information the enzyme is required for the regulation of the level of dolichyl phosphate and thus the rate of glycoprotein synthesis Tetrahymena pyriformis ?
-
 ?
3.1.3.51 additional information the enzyme is required for the regulation of the level of dolichyl phosphate and thus the rate of glycoprotein synthesis Tetrahymena pyriformis WH14 ?
-
 ?
3.1.3.51 p-nitrophenyl phosphate + H2O
-
 Tetrahymena pyriformis p-nitrophenol + phosphate
-
 ?
3.1.3.51 p-nitrophenyl phosphate + H2O
-
 Tetrahymena pyriformis WH14 p-nitrophenol + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.51 40
-
 reaction rate increases up to Tetrahymena pyriformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.51 50
-
 10 min, 75% loss of activity Tetrahymena pyriformis
3.1.3.51 60
-
 10 min, complete loss of activity Tetrahymena pyriformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.51 5 6
-
 Tetrahymena pyriformis