BRENDA - Enzyme Database

A dolichyl phosphate-cleaving acid phosphatase from Tetrahymena pyriformis

Adrian, G.S.; Keenan, R.W.; Biochim. Biophys. Acta 575, 431-438 (1979)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.51
Ethoxy formic anhydride
10 mM, 67% inhibition of dolichyl phosphate hydrolysis, no inhibition of p-nitrophenyl phosphate cleavage
Tetrahymena pyriformis
3.1.3.51
Tartrate
inhibits hydrolysis of p-nitrophenyl phosphate, no inhibition of the hydrolysis of dolichyl phosphate
Tetrahymena pyriformis
3.1.3.51
vanadium oxide
inhibits hydrolysis of p-nitrophenyl phosphate and dolichyl phosphate
Tetrahymena pyriformis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.51
Mg2+
hydrolysis of dolichyl phosphate requires Mg2+
Tetrahymena pyriformis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.51
additional information
Tetrahymena pyriformis
the enzyme is required for the regulation of the level of dolichyl phosphate and thus the rate of glycoprotein synthesis
?
-
-
-
3.1.3.51
additional information
Tetrahymena pyriformis WH14
the enzyme is required for the regulation of the level of dolichyl phosphate and thus the rate of glycoprotein synthesis
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.51
Tetrahymena pyriformis
-
-
-
3.1.3.51
Tetrahymena pyriformis WH14
-
-
-
Storage Stability
EC Number
Storage Stability
Organism
3.1.3.51
-20°C, stable for several months
Tetrahymena pyriformis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.51
dolichyl phosphate + H2O
-
134907
Tetrahymena pyriformis
dolichol + phosphate
-
-
-
?
3.1.3.51
dolichyl phosphate + H2O
-
134907
Tetrahymena pyriformis WH14
dolichol + phosphate
-
-
-
?
3.1.3.51
additional information
the enzyme is required for the regulation of the level of dolichyl phosphate and thus the rate of glycoprotein synthesis
134907
Tetrahymena pyriformis
?
-
-
-
-
3.1.3.51
additional information
the enzyme is required for the regulation of the level of dolichyl phosphate and thus the rate of glycoprotein synthesis
134907
Tetrahymena pyriformis WH14
?
-
-
-
-
3.1.3.51
p-nitrophenyl phosphate + H2O
-
134907
Tetrahymena pyriformis
p-nitrophenol + phosphate
-
-
-
?
3.1.3.51
p-nitrophenyl phosphate + H2O
-
134907
Tetrahymena pyriformis WH14
p-nitrophenol + phosphate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.51
40
-
reaction rate increases up to
Tetrahymena pyriformis
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.51
50
-
10 min, 75% loss of activity
Tetrahymena pyriformis
3.1.3.51
60
-
10 min, complete loss of activity
Tetrahymena pyriformis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.51
5
6
-
Tetrahymena pyriformis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.51
Ethoxy formic anhydride
10 mM, 67% inhibition of dolichyl phosphate hydrolysis, no inhibition of p-nitrophenyl phosphate cleavage
Tetrahymena pyriformis
3.1.3.51
Tartrate
inhibits hydrolysis of p-nitrophenyl phosphate, no inhibition of the hydrolysis of dolichyl phosphate
Tetrahymena pyriformis
3.1.3.51
vanadium oxide
inhibits hydrolysis of p-nitrophenyl phosphate and dolichyl phosphate
Tetrahymena pyriformis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.51
Mg2+
hydrolysis of dolichyl phosphate requires Mg2+
Tetrahymena pyriformis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.51
additional information
Tetrahymena pyriformis
the enzyme is required for the regulation of the level of dolichyl phosphate and thus the rate of glycoprotein synthesis
?
-
-
-
3.1.3.51
additional information
Tetrahymena pyriformis WH14
the enzyme is required for the regulation of the level of dolichyl phosphate and thus the rate of glycoprotein synthesis
?
-
-
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.3.51
-20°C, stable for several months
Tetrahymena pyriformis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.51
dolichyl phosphate + H2O
-
134907
Tetrahymena pyriformis
dolichol + phosphate
-
-
-
?
3.1.3.51
dolichyl phosphate + H2O
-
134907
Tetrahymena pyriformis WH14
dolichol + phosphate
-
-
-
?
3.1.3.51
additional information
the enzyme is required for the regulation of the level of dolichyl phosphate and thus the rate of glycoprotein synthesis
134907
Tetrahymena pyriformis
?
-
-
-
-
3.1.3.51
additional information
the enzyme is required for the regulation of the level of dolichyl phosphate and thus the rate of glycoprotein synthesis
134907
Tetrahymena pyriformis WH14
?
-
-
-
-
3.1.3.51
p-nitrophenyl phosphate + H2O
-
134907
Tetrahymena pyriformis
p-nitrophenol + phosphate
-
-
-
?
3.1.3.51
p-nitrophenyl phosphate + H2O
-
134907
Tetrahymena pyriformis WH14
p-nitrophenol + phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.51
40
-
reaction rate increases up to
Tetrahymena pyriformis
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.51
50
-
10 min, 75% loss of activity
Tetrahymena pyriformis
3.1.3.51
60
-
10 min, complete loss of activity
Tetrahymena pyriformis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.51
5
6
-
Tetrahymena pyriformis