BRENDA - Enzyme Database

Characterization and purification of neutrophil ecto-phosphatidic acid phosphohydrolase

English, D.; Martin, M.; Harvea, K.A.; Akard, L.P.; Allen, R.; Widlanski, T.S.; Garcia, J.G.; Siddiqui, R.A.; Biochem. J. 324, 941-950 (1997)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
dihydrosphingosine
-
Homo sapiens
3.1.3.4
dimethylsphingosine
-
Homo sapiens
3.1.3.4
F-
NaF
Homo sapiens
3.1.3.4
Mg2+
MgCl2
Homo sapiens
3.1.3.4
NEM
no inhibition
Homo sapiens
3.1.3.4
phosphatidic acid
competitive inhibition
Homo sapiens
3.1.3.4
propanolol
-
Homo sapiens
3.1.3.4
sphingosine
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
1.4
-
dioctanoyl phosphatidic acid
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
plasma membrane
-
Homo sapiens
5886
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
additional information
Homo sapiens
the enzyme may play an important role in regulating inflammatory cell responses to extracellular phosphatidic acid in biological system
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.4
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
neutrophil
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.4
0.1175
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
didecanoyl phosphatidic acid + H2O
-
134897
Homo sapiens
1,2-didecanoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
dihexanoyl phosphatidic acid + H2O
-
134897
Homo sapiens
1,2-dihexanoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
dimyristoyl phosphatidic acid + H2O
-
134897
Homo sapiens
1,2-dimyristoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
dioctanoyl phosphatidic acid + H2O
-
134897
Homo sapiens
1,2-dioctanoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
dioleoyl phosphatidic acid + H2O
-
134897
Homo sapiens
1,2-dioleoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
dipalmitoyl phosphatidic acid + H2O
-
134897
Homo sapiens
?
-
-
-
?
3.1.3.4
lysophosphatidate + H2O
-
134897
Homo sapiens
?
-
-
-
?
3.1.3.4
additional information
both long and short chain lysophosphatidic acids are hydrolyzed at rates comparable with that observed for short chain phosphatidic acids
134897
Homo sapiens
?
-
-
-
-
3.1.3.4
additional information
the enzyme may play an important role in regulating inflammatory cell responses to extracellular phosphatidic acid in biological system
134897
Homo sapiens
?
-
-
-
-
3.1.3.4
octanoyl lysophosphatidic acid + H2O
-
134897
Homo sapiens
?
-
-
-
?
3.1.3.4
oleoyl phosphatidic acid + H2O
-
134897
Homo sapiens
?
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
134897
Homo sapiens
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.4
?
x * 45000-52000, SDS-PAGE
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
dihydrosphingosine
-
Homo sapiens
3.1.3.4
dimethylsphingosine
-
Homo sapiens
3.1.3.4
F-
NaF
Homo sapiens
3.1.3.4
Mg2+
MgCl2
Homo sapiens
3.1.3.4
NEM
no inhibition
Homo sapiens
3.1.3.4
phosphatidic acid
competitive inhibition
Homo sapiens
3.1.3.4
propanolol
-
Homo sapiens
3.1.3.4
sphingosine
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
1.4
-
dioctanoyl phosphatidic acid
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
plasma membrane
-
Homo sapiens
5886
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
additional information
Homo sapiens
the enzyme may play an important role in regulating inflammatory cell responses to extracellular phosphatidic acid in biological system
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
neutrophil
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.4
0.1175
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
didecanoyl phosphatidic acid + H2O
-
134897
Homo sapiens
1,2-didecanoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
dihexanoyl phosphatidic acid + H2O
-
134897
Homo sapiens
1,2-dihexanoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
dimyristoyl phosphatidic acid + H2O
-
134897
Homo sapiens
1,2-dimyristoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
dioctanoyl phosphatidic acid + H2O
-
134897
Homo sapiens
1,2-dioctanoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
dioleoyl phosphatidic acid + H2O
-
134897
Homo sapiens
1,2-dioleoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
dipalmitoyl phosphatidic acid + H2O
-
134897
Homo sapiens
?
-
-
-
?
3.1.3.4
lysophosphatidate + H2O
-
134897
Homo sapiens
?
-
-
-
?
3.1.3.4
additional information
both long and short chain lysophosphatidic acids are hydrolyzed at rates comparable with that observed for short chain phosphatidic acids
134897
Homo sapiens
?
-
-
-
-
3.1.3.4
additional information
the enzyme may play an important role in regulating inflammatory cell responses to extracellular phosphatidic acid in biological system
134897
Homo sapiens
?
-
-
-
-
3.1.3.4
octanoyl lysophosphatidic acid + H2O
-
134897
Homo sapiens
?
-
-
-
?
3.1.3.4
oleoyl phosphatidic acid + H2O
-
134897
Homo sapiens
?
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
134897
Homo sapiens
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.4
?
x * 45000-52000, SDS-PAGE
Homo sapiens