BRENDA - Enzyme Database

Purification and characterization of N-ethylmaleimide-insensitive phosphatidic acid phosphohydrolase (PAP2) from rat liver

Fleming, I.N.; Yeaman, S.J.; Biochem. J. 308, 983-989 (1995)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
1,2-diacylglycerol
-
Rattus norvegicus
3.1.3.4
amphiphilic amines
-
Rattus norvegicus
3.1.3.4
F-
enzyme form PAP2
Rattus norvegicus
3.1.3.4
Mn2+
-
Rattus norvegicus
3.1.3.4
NEM
no inhibition
Rattus norvegicus
3.1.3.4
propanolol
partial
Rattus norvegicus
3.1.3.4
Zn2+
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.032
-
phosphatidic acid
enzyme form PAP2B
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
38000
-
x * 38000, enzyme form PAP2B, SDS-PAGE
Rattus norvegicus
3.1.3.4
265000
-
native PAGE
Rattus norvegicus
3.1.3.4
290000
300000
gel filtration
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.4
two forms of PAP2: PAP2A and PAP2B
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.4
10
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate + H2O
-
134896
Rattus norvegicus
1-stearoyl-2-arachidonoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
didecanoyl phosphatidic acid + H2O
-
134896
Rattus norvegicus
1,2-didecanoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
dihexanoyl phosphatidic acid + H2O
-
134896
Rattus norvegicus
1,2-dihexanoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
dimyristoyl phosphatidic acid + H2O
-
134896
Rattus norvegicus
1,2-dimyristoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
dioleoyl phosphatidic acid + H2O
-
134896
Rattus norvegicus
1,2-dioleoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
distearoyl phosphatidic acid + H2O
-
134896
Rattus norvegicus
1,2-distearoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
134896
Rattus norvegicus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.4
?
x * 38000, enzyme form PAP2B, SDS-PAGE
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
1,2-diacylglycerol
-
Rattus norvegicus
3.1.3.4
amphiphilic amines
-
Rattus norvegicus
3.1.3.4
F-
enzyme form PAP2
Rattus norvegicus
3.1.3.4
Mn2+
-
Rattus norvegicus
3.1.3.4
NEM
no inhibition
Rattus norvegicus
3.1.3.4
propanolol
partial
Rattus norvegicus
3.1.3.4
Zn2+
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.032
-
phosphatidic acid
enzyme form PAP2B
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
38000
-
x * 38000, enzyme form PAP2B, SDS-PAGE
Rattus norvegicus
3.1.3.4
265000
-
native PAGE
Rattus norvegicus
3.1.3.4
290000
300000
gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
two forms of PAP2: PAP2A and PAP2B
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.4
10
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate + H2O
-
134896
Rattus norvegicus
1-stearoyl-2-arachidonoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
didecanoyl phosphatidic acid + H2O
-
134896
Rattus norvegicus
1,2-didecanoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
dihexanoyl phosphatidic acid + H2O
-
134896
Rattus norvegicus
1,2-dihexanoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
dimyristoyl phosphatidic acid + H2O
-
134896
Rattus norvegicus
1,2-dimyristoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
dioleoyl phosphatidic acid + H2O
-
134896
Rattus norvegicus
1,2-dioleoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
distearoyl phosphatidic acid + H2O
-
134896
Rattus norvegicus
1,2-distearoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
134896
Rattus norvegicus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.4
?
x * 38000, enzyme form PAP2B, SDS-PAGE
Rattus norvegicus