BRENDA - Enzyme Database

Phosphatidate phosphatase from Saccharomyces cerevisiae. Isolation of 45- and 104-kDa forms of the enzyme that are differentially regulated by inositol

Morlock, K.R.; McLaughlin, J.J.; Lin, Y.P.; Carman, G.M.; J. Biol. Chem. 266, 3586-3593 (1991)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
Triton X-100
the 45000 Da enzyme form shows maximal activity at 5 mM
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
NEM
45000 Da enzyme form, at 1 mM
Saccharomyces cerevisiae
3.1.3.4
p-chloromercuriphenylsulfonic acid
45000 Da enzyme form, at 1 mM
Saccharomyces cerevisiae
3.1.3.4
Phenylglyoxal
inhibition of the 45000 Da enzyme form and the 104000 Da enzyme form
Saccharomyces cerevisiae
3.1.3.4
propanolol
inhibition of the 45000 Da enzyme form and the 104000 Da enzyme form
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
microsome
-
Saccharomyces cerevisiae
-
-
3.1.3.4
mitochondrion
-
Saccharomyces cerevisiae
5739
-
3.1.3.4
plasma membrane
associated
Saccharomyces cerevisiae
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
required for the 45000 Da enzyme form, maximal activity at 1 mM
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
additional information
Saccharomyces cerevisiae
the 45000 Da enzyme form and 104000 Da enzyme form are induced when cells enter the stationary phase of growth
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.4
45000 Da enzyme form and 104000 Da enzyme form
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.3.4
2.35
-
-
Saccharomyces cerevisiae
Storage Stability
EC Number
Storage Stability
Organism
3.1.3.4
-80░C, the 45000 Da enzyme form is stable for 3 months, the 104000 Da enzyme form is degraded to a 91000 Da enzyme form and retains full activity
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
additional information
the 45000 Da enzyme form and 104000 Da enzyme form are induced when cells enter the stationary phase of growth
134892
Saccharomyces cerevisiae
?
-
-
-
-
3.1.3.4
phosphatidic acid + H2O
-
134892
Saccharomyces cerevisiae
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.1.3.4
30
-
45000 Da enzyme form, unstable above
Saccharomyces cerevisiae
3.1.3.4
50
-
45000 Da enzyme form, complete inactivation after 20 min
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
6
7
45000 Da enzyme form
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
Triton X-100
the 45000 Da enzyme form shows maximal activity at 5 mM
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
NEM
45000 Da enzyme form, at 1 mM
Saccharomyces cerevisiae
3.1.3.4
p-chloromercuriphenylsulfonic acid
45000 Da enzyme form, at 1 mM
Saccharomyces cerevisiae
3.1.3.4
Phenylglyoxal
inhibition of the 45000 Da enzyme form and the 104000 Da enzyme form
Saccharomyces cerevisiae
3.1.3.4
propanolol
inhibition of the 45000 Da enzyme form and the 104000 Da enzyme form
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
microsome
-
Saccharomyces cerevisiae
-
-
3.1.3.4
mitochondrion
-
Saccharomyces cerevisiae
5739
-
3.1.3.4
plasma membrane
associated
Saccharomyces cerevisiae
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
required for the 45000 Da enzyme form, maximal activity at 1 mM
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
additional information
Saccharomyces cerevisiae
the 45000 Da enzyme form and 104000 Da enzyme form are induced when cells enter the stationary phase of growth
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
45000 Da enzyme form and 104000 Da enzyme form
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.3.4
2.35
-
-
Saccharomyces cerevisiae
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.3.4
-80░C, the 45000 Da enzyme form is stable for 3 months, the 104000 Da enzyme form is degraded to a 91000 Da enzyme form and retains full activity
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
additional information
the 45000 Da enzyme form and 104000 Da enzyme form are induced when cells enter the stationary phase of growth
134892
Saccharomyces cerevisiae
?
-
-
-
-
3.1.3.4
phosphatidic acid + H2O
-
134892
Saccharomyces cerevisiae
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.1.3.4
30
-
45000 Da enzyme form, unstable above
Saccharomyces cerevisiae
3.1.3.4
50
-
45000 Da enzyme form, complete inactivation after 20 min
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
6
7
45000 Da enzyme form
Saccharomyces cerevisiae