BRENDA - Enzyme Database

Purification of a phosphatase which hydrolyzes phosphatidic acid, a key intermediate in glucolipid synthesis in Acholeplasma laidlawii A membranes

Berg, S.; Wieslander, A.; Biochim. Biophys. Acta 1330, 225-232 (1997)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
Cd2+
-
Acholeplasma laidlawii
3.1.3.4
Hg2+
-
Acholeplasma laidlawii
3.1.3.4
p-nitrophenyl phosphate
inhibits hydrolysis of phosphatidic acid
Acholeplasma laidlawii
3.1.3.4
Zn2+
-
Acholeplasma laidlawii
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
integral to plasma membrane
-
Acholeplasma laidlawii
-
-
3.1.3.4
plasma membrane
-
Acholeplasma laidlawii
5886
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
25000
-
x * 25000, SDS-PAGE
Acholeplasma laidlawii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
additional information
Acholeplasma laidlawii
the enzyme is proposed to catalyze the first enzymatic step in the important glucolipid pathway
?
-
-
-
3.1.3.4
additional information
Acholeplasma laidlawii A
the enzyme is proposed to catalyze the first enzymatic step in the important glucolipid pathway
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Acholeplasma laidlawii
-
A
-
3.1.3.4
Acholeplasma laidlawii A
-
A
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.4
-
Acholeplasma laidlawii
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.3.4
12.78
-
-
Acholeplasma laidlawii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
additional information
the enzyme is proposed to catalyze the first enzymatic step in the important glucolipid pathway
134891
Acholeplasma laidlawii
?
-
-
-
-
3.1.3.4
additional information
the enzyme is proposed to catalyze the first enzymatic step in the important glucolipid pathway
134891
Acholeplasma laidlawii A
?
-
-
-
-
3.1.3.4
phosphatidic acid + H2O
-
134891
Acholeplasma laidlawii
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
134891
Acholeplasma laidlawii A
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.4
?
x * 25000, SDS-PAGE
Acholeplasma laidlawii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
8
-
dephosphorylation of phosphatidic acid and p-nitrophenyl phosphate
Acholeplasma laidlawii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
Cd2+
-
Acholeplasma laidlawii
3.1.3.4
Hg2+
-
Acholeplasma laidlawii
3.1.3.4
p-nitrophenyl phosphate
inhibits hydrolysis of phosphatidic acid
Acholeplasma laidlawii
3.1.3.4
Zn2+
-
Acholeplasma laidlawii
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
integral to plasma membrane
-
Acholeplasma laidlawii
-
-
3.1.3.4
plasma membrane
-
Acholeplasma laidlawii
5886
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
25000
-
x * 25000, SDS-PAGE
Acholeplasma laidlawii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
additional information
Acholeplasma laidlawii
the enzyme is proposed to catalyze the first enzymatic step in the important glucolipid pathway
?
-
-
-
3.1.3.4
additional information
Acholeplasma laidlawii A
the enzyme is proposed to catalyze the first enzymatic step in the important glucolipid pathway
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
-
Acholeplasma laidlawii
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.3.4
12.78
-
-
Acholeplasma laidlawii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
additional information
the enzyme is proposed to catalyze the first enzymatic step in the important glucolipid pathway
134891
Acholeplasma laidlawii
?
-
-
-
-
3.1.3.4
additional information
the enzyme is proposed to catalyze the first enzymatic step in the important glucolipid pathway
134891
Acholeplasma laidlawii A
?
-
-
-
-
3.1.3.4
phosphatidic acid + H2O
-
134891
Acholeplasma laidlawii
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
134891
Acholeplasma laidlawii A
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.4
?
x * 25000, SDS-PAGE
Acholeplasma laidlawii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
8
-
dephosphorylation of phosphatidic acid and p-nitrophenyl phosphate
Acholeplasma laidlawii