BRENDA - Enzyme Database

Purification and properties of phosphatidic acid phosphatase from porcine thymus membranes

Kanoh, H.; Imai, S.; Yamada, K.; Sakane, F.; J. Biol. Chem. 267, 25309-25314 (1992)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
deoxycholate
Triton X-100 or deoxycholate required
Sus scrofa
3.1.3.4
Triton X-100
Triton X-100 or deoxycholate required
Sus scrofa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
1,2-diacylglycerol
-
Sus scrofa
3.1.3.4
Ca2+
-
Sus scrofa
3.1.3.4
chlorpromazine
-
Sus scrofa
3.1.3.4
F-
NaF
Sus scrofa
3.1.3.4
Mn2+
-
Sus scrofa
3.1.3.4
NEM
no inhibition
Sus scrofa
3.1.3.4
propanolol
-
Sus scrofa
3.1.3.4
sphingosine
-
Sus scrofa
3.1.3.4
Zn2+
-
Sus scrofa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
integral to plasma membrane
-
Sus scrofa
-
-
3.1.3.4
plasma membrane
-
Sus scrofa
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
independent of Mg2+
Sus scrofa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
83000
-
x * 83000, SDS-PAGE under reducing and nonreducing conditions
Sus scrofa
3.1.3.4
218000
-
gel filtration
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.4
-
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
thymus
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.4
15.8
-
-
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
lysophosphatidate + H2O
poor substrate
134886
Sus scrofa
?
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
134886
Sus scrofa
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.4
?
x * 83000, SDS-PAGE under reducing and nonreducing conditions
Sus scrofa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
7
7.4
-
Sus scrofa
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
deoxycholate
Triton X-100 or deoxycholate required
Sus scrofa
3.1.3.4
Triton X-100
Triton X-100 or deoxycholate required
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
1,2-diacylglycerol
-
Sus scrofa
3.1.3.4
Ca2+
-
Sus scrofa
3.1.3.4
chlorpromazine
-
Sus scrofa
3.1.3.4
F-
NaF
Sus scrofa
3.1.3.4
Mn2+
-
Sus scrofa
3.1.3.4
NEM
no inhibition
Sus scrofa
3.1.3.4
propanolol
-
Sus scrofa
3.1.3.4
sphingosine
-
Sus scrofa
3.1.3.4
Zn2+
-
Sus scrofa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
integral to plasma membrane
-
Sus scrofa
-
-
3.1.3.4
plasma membrane
-
Sus scrofa
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
independent of Mg2+
Sus scrofa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
83000
-
x * 83000, SDS-PAGE under reducing and nonreducing conditions
Sus scrofa
3.1.3.4
218000
-
gel filtration
Sus scrofa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
-
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
thymus
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.4
15.8
-
-
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
lysophosphatidate + H2O
poor substrate
134886
Sus scrofa
?
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
134886
Sus scrofa
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.4
?
x * 83000, SDS-PAGE under reducing and nonreducing conditions
Sus scrofa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
7
7.4
-
Sus scrofa