BRENDA - Enzyme Database

Human type 2 phosphatidic acid phosphohydrolases. Substrate specificity of the type 2a, 2b, and 2c enzymes and cell surface activity of the 2a isoform

Roberts, R.; Sciorra, V.A.; Morris, A.J.; J. Biol. Chem. 273, 22059-22067 (1998)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
expression of hemagglutinin epitope-tagged phosphatidic acid phosphohydrolase PAP-2a, PAP-2b and PAP-2c in HEK293 cells. Expression of phosphatidic acid phosphohydrolase PAP-2a, PAP-2b and PAP-2c in baculovirus-infected Sf9 insect cells. Expression of PAP-2a but not PAP-2b or PAP-2c results in high levels of cell surface PAP activity in intact insect cells
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
propanolol
effective inhibitor of type 1 phosphatidic acid phosphatase, modest inhibitor of phosphatidic acid phosphatase PAP-2a, PAP-2b and PAP-2c
Homo sapiens
3.1.3.4
sphingosine
-
Homo sapiens
3.1.3.4
Zn2+
-
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Homo sapiens
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
-
134881
Homo sapiens
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
expression of hemagglutinin epitope-tagged phosphatidic acid phosphohydrolase PAP-2a, PAP-2b and PAP-2c in HEK293 cells. Expression of phosphatidic acid phosphohydrolase PAP-2a, PAP-2b and PAP-2c in baculovirus-infected Sf9 insect cells. Expression of PAP-2a but not PAP-2b or PAP-2c results in high levels of cell surface PAP activity in intact insect cells
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
propanolol
effective inhibitor of type 1 phosphatidic acid phosphatase, modest inhibitor of phosphatidic acid phosphatase PAP-2a, PAP-2b and PAP-2c
Homo sapiens
3.1.3.4
sphingosine
-
Homo sapiens
3.1.3.4
Zn2+
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
-
134881
Homo sapiens
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?