BRENDA - Enzyme Database

Partial purification, properties, and subcellular distribution of rat liver phosphatidate phosphatase

Hosaka, K.; Yamashita, S.; Numa, S.; J. Biochem. 77, 501-509 (1975)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
phosphatidylcholine
stimulation
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
F-
50 mM, 90% inhibition
Rattus norvegicus
3.1.3.4
PCMB
0.05 mM, 90% inhibition
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.05
-
phosphatidic acid
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytosol
the largest portion of the activity is localized in the soluble fraction
Rattus norvegicus
5829
-
3.1.3.4
lysosome
only 3% of the total activity
Rattus norvegicus
5764
-
3.1.3.4
microsome
-
Rattus norvegicus
-
-
3.1.3.4
mitochondrion
-
Rattus norvegicus
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
required
Rattus norvegicus
3.1.3.4
Mn2+
slight stimulation
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.4
partial
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.4
165
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
1,2-dipalmitoyl-sn-glycerol 3-phosphate + H2O
-
134879
Rattus norvegicus
1,2-dipalmitoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
1-acyl-sn-glycerol 3-phosphate + H2O
at 9.1% of the activity with phosphatidic acid
134879
Rattus norvegicus
1-acyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
5'-AMP + H2O
8.6% of the activity with phosphatidic acid
134879
Rattus norvegicus
?
-
-
-
?
3.1.3.4
ATP + H2O
7.2% of the activity with phosphatidic acid
134879
Rattus norvegicus
?
-
-
-
?
3.1.3.4
Glucose 6-phosphate + H2O
at 5.2% of the activity with phosphatidic acid
134879
Rattus norvegicus
Glucose + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
134879
Rattus norvegicus
1,2-diacyl-sn-glycerol + phosphate
-
134879
Rattus norvegicus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
7.4
7.6
-
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.4
7.2
8.2
pH 7.2: 76% of maximal activity, pH 8.2: 74% of maximal activity
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
phosphatidylcholine
stimulation
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
F-
50 mM, 90% inhibition
Rattus norvegicus
3.1.3.4
PCMB
0.05 mM, 90% inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.05
-
phosphatidic acid
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytosol
the largest portion of the activity is localized in the soluble fraction
Rattus norvegicus
5829
-
3.1.3.4
lysosome
only 3% of the total activity
Rattus norvegicus
5764
-
3.1.3.4
microsome
-
Rattus norvegicus
-
-
3.1.3.4
mitochondrion
-
Rattus norvegicus
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
required
Rattus norvegicus
3.1.3.4
Mn2+
slight stimulation
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
partial
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.4
165
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
1,2-dipalmitoyl-sn-glycerol 3-phosphate + H2O
-
134879
Rattus norvegicus
1,2-dipalmitoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
1-acyl-sn-glycerol 3-phosphate + H2O
at 9.1% of the activity with phosphatidic acid
134879
Rattus norvegicus
1-acyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
5'-AMP + H2O
8.6% of the activity with phosphatidic acid
134879
Rattus norvegicus
?
-
-
-
?
3.1.3.4
ATP + H2O
7.2% of the activity with phosphatidic acid
134879
Rattus norvegicus
?
-
-
-
?
3.1.3.4
Glucose 6-phosphate + H2O
at 5.2% of the activity with phosphatidic acid
134879
Rattus norvegicus
Glucose + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
134879
Rattus norvegicus
1,2-diacyl-sn-glycerol + phosphate
-
134879
Rattus norvegicus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
7.4
7.6
-
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.4
7.2
8.2
pH 7.2: 76% of maximal activity, pH 8.2: 74% of maximal activity
Rattus norvegicus