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Literature summary extracted from

  • Hosaka, K.; Yamashita, S.; Numa, S.
    Partial purification, properties, and subcellular distribution of rat liver phosphatidate phosphatase (1975), J. Biochem., 77, 501-509.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.4 phosphatidylcholine stimulation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.4 F- 50 mM, 90% inhibition Rattus norvegicus
3.1.3.4 PCMB 0.05 mM, 90% inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.4 0.05
-
 phosphatidic acid
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.4 cytosol the largest portion of the activity is localized in the soluble fraction Rattus norvegicus 5829
-
3.1.3.4 lysosome only 3% of the total activity Rattus norvegicus 5764
-
3.1.3.4 microsome
-
 Rattus norvegicus
-
-
3.1.3.4 mitochondrion
-
 Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.4 Mg2+ required Rattus norvegicus
3.1.3.4 Mn2+ slight stimulation Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.4 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.4 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.4 165
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.4 1,2-dipalmitoyl-sn-glycerol 3-phosphate + H2O
-
 Rattus norvegicus 1,2-dipalmitoyl-sn-glycerol + phosphate
-
 ?
3.1.3.4 1-acyl-sn-glycerol 3-phosphate + H2O at 9.1% of the activity with phosphatidic acid Rattus norvegicus 1-acyl-sn-glycerol + phosphate
-
 ?
3.1.3.4 5'-AMP + H2O 8.6% of the activity with phosphatidic acid Rattus norvegicus ?
-
 ?
3.1.3.4 ATP + H2O 7.2% of the activity with phosphatidic acid Rattus norvegicus ?
-
 ?
3.1.3.4 Glucose 6-phosphate + H2O at 5.2% of the activity with phosphatidic acid Rattus norvegicus Glucose + phosphate
-
 ?
3.1.3.4 phosphatidic acid + H2O
-
 Rattus norvegicus 1,2-diacyl-sn-glycerol + phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.4 7.4 7.6
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.4 7.2 8.2 pH 7.2: 76% of maximal activity, pH 8.2: 74% of maximal activity Rattus norvegicus