BRENDA - Enzyme Database

Pulmonary phosphatidic acid phosphatase. Properties of membrane-bound phosphatidate-dependent phosphatidic acid phosphatase in rat lung

Casola, P.G.; Possmayer, F.; Biochim. Biophys. Acta 574, 212-225 (1979)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
EDTA
1.0-2.0 mM, complete loss of cytosolic activity, reduced microsomal activity
Rattus norvegicus
3.1.3.4
Mg2+
above 5 mM
Rattus norvegicus
3.1.3.4
phosphatidylcholine
slight inhibition of cytosolic activity
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.0054
-
phosphatidic acid
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytosol
-
Rattus norvegicus
5829
-
3.1.3.4
microsome
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
below 5 mM, slight stimulation of cytosolic activity, inhibition at higher concentrations
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
additional information
Rattus norvegicus
the activity associated with the cytosol has a role in phosphocholine biosynthesis in rat lung
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
lung
-
Rattus norvegicus
-
Storage Stability
EC Number
Storage Stability
Organism
3.1.3.4
-70°C, activity in cytosolic fraction is stable for at least two months
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
additional information
the activity associated with the cytosol has a role in phosphocholine biosynthesis in rat lung
134874
Rattus norvegicus
?
-
-
-
-
3.1.3.4
phosphatidic acid + H2O
-
134874
Rattus norvegicus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
6.5
7.5
membrane-bound phosphatidic acid, microsomal enzyme
Rattus norvegicus
3.1.3.4
7.4
8
membrane-bound phosphatidic acid, cytosolic enzyme
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
EDTA
1.0-2.0 mM, complete loss of cytosolic activity, reduced microsomal activity
Rattus norvegicus
3.1.3.4
Mg2+
above 5 mM
Rattus norvegicus
3.1.3.4
phosphatidylcholine
slight inhibition of cytosolic activity
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.0054
-
phosphatidic acid
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytosol
-
Rattus norvegicus
5829
-
3.1.3.4
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
below 5 mM, slight stimulation of cytosolic activity, inhibition at higher concentrations
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
additional information
Rattus norvegicus
the activity associated with the cytosol has a role in phosphocholine biosynthesis in rat lung
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
lung
-
Rattus norvegicus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.3.4
-70°C, activity in cytosolic fraction is stable for at least two months
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
additional information
the activity associated with the cytosol has a role in phosphocholine biosynthesis in rat lung
134874
Rattus norvegicus
?
-
-
-
-
3.1.3.4
phosphatidic acid + H2O
-
134874
Rattus norvegicus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
6.5
7.5
membrane-bound phosphatidic acid, microsomal enzyme
Rattus norvegicus
3.1.3.4
7.4
8
membrane-bound phosphatidic acid, cytosolic enzyme
Rattus norvegicus