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Literature summary extracted from

  • Casola, P.G.; Possmayer, F.
    Pulmonary phosphatidic acid phosphatase. Properties of membrane-bound phosphatidate-dependent phosphatidic acid phosphatase in rat lung (1979), Biochim. Biophys. Acta, 574, 212-225.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.4 EDTA 1.0-2.0 mM, complete loss of cytosolic activity, reduced microsomal activity Rattus norvegicus
3.1.3.4 Mg2+ above 5 mM Rattus norvegicus
3.1.3.4 phosphatidylcholine slight inhibition of cytosolic activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.4 0.0054
-
 phosphatidic acid
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.4 cytosol
-
 Rattus norvegicus 5829
-
3.1.3.4 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.4 Mg2+ below 5 mM, slight stimulation of cytosolic activity, inhibition at higher concentrations Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.4 additional information Rattus norvegicus the activity associated with the cytosol has a role in phosphocholine biosynthesis in rat lung ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.4 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.4 lung
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
3.1.3.4 -70°C, activity in cytosolic fraction is stable for at least two months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.4 additional information the activity associated with the cytosol has a role in phosphocholine biosynthesis in rat lung Rattus norvegicus ?
-
 ?
3.1.3.4 phosphatidic acid + H2O
-
 Rattus norvegicus 1,2-diacyl-sn-glycerol + phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.4 6.5 7.5 membrane-bound phosphatidic acid, microsomal enzyme Rattus norvegicus
3.1.3.4 7.4 8 membrane-bound phosphatidic acid, cytosolic enzyme Rattus norvegicus