BRENDA - Enzyme Database

Separation and characterization of the membrane-bound and aqueously dispersed phosphatidate phosphatidic acid phosphohydrolase activities in rat lung

Casola, P.G.; Possmayer, F.; Biochim. Biophys. Acta 664, 298-315 (1981)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
Triton X-100
5.5fold increase in activity with membrane-bound phosphatidic acid
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
EDTA
inhibits activity with membrane-bound phosphatidic acid in the cytosol by 65%, the activity with aqueously dispersed phosphatidic acid is inhibited 10%
Rattus norvegicus
3.1.3.4
Triton X-100
slight depression of activity with aqueously dispersed phosphatidic acid
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytosol
-
Rattus norvegicus
5829
-
3.1.3.4
microsome
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
MgCl2 stimulates activity with membrane-bound phosphatidic acid in the cytosol by 7%, the activity with aqueously dispersed phosphatidic acid is inhibited 80%
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
lung
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
membrane-bound phosphatidic acid and aqueously dispersed phosphatidic acid
134872
Rattus norvegicus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.4
55
-
20 min, 70% loss of activity with membrane-bound phosphatidic acid, 24% loss of activity with aqueously dispersed phosphatidic acid
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
Triton X-100
5.5fold increase in activity with membrane-bound phosphatidic acid
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
EDTA
inhibits activity with membrane-bound phosphatidic acid in the cytosol by 65%, the activity with aqueously dispersed phosphatidic acid is inhibited 10%
Rattus norvegicus
3.1.3.4
Triton X-100
slight depression of activity with aqueously dispersed phosphatidic acid
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytosol
-
Rattus norvegicus
5829
-
3.1.3.4
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
MgCl2 stimulates activity with membrane-bound phosphatidic acid in the cytosol by 7%, the activity with aqueously dispersed phosphatidic acid is inhibited 80%
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
lung
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
membrane-bound phosphatidic acid and aqueously dispersed phosphatidic acid
134872
Rattus norvegicus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.4
55
-
20 min, 70% loss of activity with membrane-bound phosphatidic acid, 24% loss of activity with aqueously dispersed phosphatidic acid
Rattus norvegicus