BRENDA - Enzyme Database

Glycerolipid biosynthesis in rat adipose tissue 12. Properties of Mg2+-dependent and -independent phosphatidate phosphohydrolase

Jamdar, S.C.; Osborne, L.J.; Wells, G.N.; Arch. Biochem. Biophys. 233, 370-377 (1984)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
NEM
the Mg2+-dependent enzyme is inhibited, the Mg2+-independent enzyme is unaffected
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
plasma membrane
-
Rattus norvegicus
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
the enzyme exists in a Mg2+-dependent enzyme form and a Mg2+-independent enzyme form
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
adipose tissue
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
-
134867
Rattus norvegicus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.4
55
-
10 min, over 80% of the Mg2+-dependent activity is lost durring incubation of cytosol and microsomal fractions, the Mg2+-independent enzyme is stable
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
NEM
the Mg2+-dependent enzyme is inhibited, the Mg2+-independent enzyme is unaffected
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
plasma membrane
-
Rattus norvegicus
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
the enzyme exists in a Mg2+-dependent enzyme form and a Mg2+-independent enzyme form
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
adipose tissue
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
-
134867
Rattus norvegicus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.4
55
-
10 min, over 80% of the Mg2+-dependent activity is lost durring incubation of cytosol and microsomal fractions, the Mg2+-independent enzyme is stable
Rattus norvegicus