BRENDA - Enzyme Database

Partial purification and properties of phosphatidate phosphatase in Saccharomyces cerevisiae

Hosaka, K.; Yamashita, S.; Biochim. Biophys. Acta 796, 102-109 (1984)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.05
-
phosphatidic acid
-
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytosol
-
Saccharomyces cerevisiae
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
required
Saccharomyces cerevisiae
3.1.3.4
Mn2+
slight stimulation
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
75000
-
gel filtration
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.4
partial
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.3.4
1.37
-
-
Saccharomyces cerevisiae
Storage Stability
EC Number
Storage Stability
Organism
3.1.3.4
-80░C, stable for at least 1 month
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
1-acyl-sn-glycerol 3-phosphate + H2O
less than 15% of the activity with phosphatidic acid
134866
Saccharomyces cerevisiae
1-acyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
highly specific for
134866
Saccharomyces cerevisiae
1,2-diacyl-sn-glycerol + phosphate
-
134866
Saccharomyces cerevisiae
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
7.5
-
-
Saccharomyces cerevisiae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.4
7
9
pH 7.0: 83% of maximal activity, pH 9.0: about 36% of maximal activity
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.05
-
phosphatidic acid
-
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytosol
-
Saccharomyces cerevisiae
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
required
Saccharomyces cerevisiae
3.1.3.4
Mn2+
slight stimulation
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
75000
-
gel filtration
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
partial
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.3.4
1.37
-
-
Saccharomyces cerevisiae
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.3.4
-80░C, stable for at least 1 month
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
1-acyl-sn-glycerol 3-phosphate + H2O
less than 15% of the activity with phosphatidic acid
134866
Saccharomyces cerevisiae
1-acyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
highly specific for
134866
Saccharomyces cerevisiae
1,2-diacyl-sn-glycerol + phosphate
-
134866
Saccharomyces cerevisiae
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
7.5
-
-
Saccharomyces cerevisiae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.4
7
9
pH 7.0: 83% of maximal activity, pH 9.0: about 36% of maximal activity
Saccharomyces cerevisiae