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Literature summary extracted from
- Partial purification and properties of phosphatidate phosphatase in Saccharomyces cerevisiae (1984), Biochim. Biophys. Acta, 796, 102-109.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.4 | 0.05 | - |
phosphatidic acid | - |
Saccharomyces cerevisiae |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.3.4 | cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.4 | Mg2+ | required | Saccharomyces cerevisiae | |
| 3.1.3.4 | Mn2+ | slight stimulation | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.4 | 75000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.4 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.4 | partial | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.4 | 1.37 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.3.4 | -80°C, stable for at least 1 month | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.4 | 1-acyl-sn-glycerol 3-phosphate + H2O | less than 15% of the activity with phosphatidic acid | Saccharomyces cerevisiae | 1-acyl-sn-glycerol + phosphate | - |
? | |
| 3.1.3.4 | phosphatidic acid + H2O | highly specific for | Saccharomyces cerevisiae | 1,2-diacyl-sn-glycerol + phosphate | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.4 | 7.5 | - |
- |
Saccharomyces cerevisiae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.4 | 7 | 9 | pH 7.0: 83% of maximal activity, pH 9.0: about 36% of maximal activity | Saccharomyces cerevisiae |
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