Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wyss, M.; Pasamontes, L.; Remy, R.; Kohler, J.; Kusznir, E.; Gadient, M.; Muller, F.; van Loon, A.P.G.M.
    Comparison of the thermostability properties of three acid phosphatases from molds: Aspergillus fumigatus phytase, A. niger phytase, and A. niger PH 2.5 acid phosphatase (1998), Appl. Environ. Microbiol., 64, 4446-4451.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Aspergillus niger
-
-
-
3.1.3.8 Aspergillus fumigatus
-
-
-
3.1.3.8 Aspergillus niger
-
-
-
3.1.3.8 Aspergillus niger T213
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.3.8 after heat denaturation at temperatures up to 90°C the enzyme refolds completely into a nativelike, fully active conformation. Capacity of the enzyme to refold properly after heat denaturation may favorably affect its pelleting stability Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Aspergillus niger 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Aspergillus fumigatus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Aspergillus niger T213 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 80
-
 denaturation and irreversible inactivation above Aspergillus niger
3.1.3.8 50
-
 20 min, about 15% loss of activity Aspergillus niger
3.1.3.8 55
-
 irreversible conformational change with loss in enzymatic activity of 70-80% Aspergillus niger
3.1.3.8 70
-
 about 75% loss of activity Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 2.5
-
-
 Aspergillus niger