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Literature summary extracted from

  • Sugiura, Y.; Kawabe, H.; Tanaka, H.; Fujimoto, S.; Ohara, A.
    Purification, enzymatic properties, and active site environment of a novel manganese(III)-containing acid phosphatase (1981), J. Biol. Chem., 256, 10664-10670.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.2
-
Ipomoea batatas

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 Acetylacetone
-
Ipomoea batatas
3.1.3.2 AsO43-
-
Ipomoea batatas
3.1.3.2 Bromosuccinimide
-
Ipomoea batatas
3.1.3.2 Cu2+
-
Ipomoea batatas
3.1.3.2 F-
-
Ipomoea batatas
3.1.3.2 Hg2+
-
Ipomoea batatas
3.1.3.2 MoO42-
-
Ipomoea batatas
3.1.3.2 PCMB
-
Ipomoea batatas
3.1.3.2 phosphate
-
Ipomoea batatas
3.1.3.2 Pyridine
-
Ipomoea batatas
3.1.3.2 Zn2+
-
Ipomoea batatas

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Mn3+ enzyme contains one atom of Mn(III) per 110000 Da enzyme Ipomoea batatas

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 55000
-
2 * 55000, SDS-PAGE Ipomoea batatas
3.1.3.2 110000
-
gel filtration Ipomoea batatas

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Ipomoea batatas
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
Ipomoea batatas

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 tuber
-
Ipomoea batatas
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 additional information
-
-
Ipomoea batatas

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 2'-AMP + H2O 6% of the activity with p-nitrophenyl phosphate Ipomoea batatas adenosine + phosphate
-
?
3.1.3.2 2-glycerophosphate + H2O 69% of the activity with p-nitrophenyl phosphate Ipomoea batatas glycerol + phosphate
-
?
3.1.3.2 3'-AMP + H2O 60% of the activity with p-nitrophenyl phosphate Ipomoea batatas adenosine + phosphate
-
?
3.1.3.2 5'-ADP + H2O 15% of the activity with p-nitrophenyl phosphate Ipomoea batatas AMP + phosphate
-
?
3.1.3.2 5'-AMP + H2O 3% of the activity with p-nitrophenyl phosphate Ipomoea batatas adenosine + phosphate
-
?
3.1.3.2 alpha-glycerophosphate + H2O 48% of the activity with p-nitrophenyl phosphate Ipomoea batatas glycerol + phosphate
-
?
3.1.3.2 AMP + H2O
-
Ipomoea batatas adenosine + phosphate
-
?
3.1.3.2 ATP + H2O 38% of the activity with p-nitrophenyl phosphate Ipomoea batatas ADP + phosphate
-
?
3.1.3.2 D-fructose 1,6-diphosphate + H2O 69% of the activity with p-nitrophenyl phosphate Ipomoea batatas ?
-
?
3.1.3.2 D-fructose 6-phosphate + H2O 29% of the activity with p-nitrophenyl phosphate Ipomoea batatas D-fructose + phosphate
-
?
3.1.3.2 D-ribose 5-phosphate + H2O 25% of the activity with p-nitrophenyl phosphate Ipomoea batatas D-ribose + phosphate
-
?
3.1.3.2 diphosphate + H2O 93% of the activity with p-nitrophenyl phosphate Ipomoea batatas 2 phosphate
-
?
3.1.3.2 FMN + H2O 35% of the activity with p-nitrophenyl phosphate Ipomoea batatas ?
-
?
3.1.3.2 Glucose 6-phosphate + H2O 33% of the activity with p-nitrophenyl phosphate Ipomoea batatas Glucose + phosphate
-
?
3.1.3.2 NADP+ + H2O 68% of the activity with p-nitrophenyl phosphate Ipomoea batatas NAD+ + phosphate
-
?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
Ipomoea batatas p-nitrophenol + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 dimer 2 * 55000, SDS-PAGE Ipomoea batatas

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 45 55 isoenzyme PI-II and PII-I Ipomoea batatas
3.1.3.2 45 50 isoenzyme PI-I Ipomoea batatas
3.1.3.2 45 60 isoenzyme PII-II Ipomoea batatas
3.1.3.2 50 60 isoenzyme PI-III and PIII-I Ipomoea batatas

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 4.5 5.5 isoenzyme PII-II Ipomoea batatas
3.1.3.2 5.5
-
-
Ipomoea batatas
3.1.3.2 5.8
-
-
Ipomoea batatas