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Literature summary extracted from

  • Hara, A.; Sawada, H.; Kato, T.; Nakayama, T.; Yamamoto, H.; Matsumoto, Y.
    Purification and characterization of a purple acid phosphatase from rat spleen (1984), J. Biochem., 95, 67-74.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.2 ascorbate maximal activation at 1 mM Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 2,2'-dipyridine
-
 Rattus norvegicus
3.1.3.2 F-
-
 Rattus norvegicus
3.1.3.2 molybdate
-
 Rattus norvegicus
3.1.3.2 o-phenanthroline
-
 Rattus norvegicus
3.1.3.2 phosphate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.62
-
 ATP
-
 Rattus norvegicus
3.1.3.2 0.87
-
 ADP
-
 Rattus norvegicus
3.1.3.2 1.1
-
 CTP
-
 Rattus norvegicus
3.1.3.2 1.1
-
 UTP
-
 Rattus norvegicus
3.1.3.2 1.2
-
 phosphoenolpyruvate
-
 Rattus norvegicus
3.1.3.2 1.2
-
 phenolphthalein monophosphate
-
 Rattus norvegicus
3.1.3.2 1.3
-
 Phenyl phosphate
-
 Rattus norvegicus
3.1.3.2 1.4
-
 1-naphthyl phosphate
-
 Rattus norvegicus
3.1.3.2 1.6
-
 Phenolphthalein diphosphate
-
 Rattus norvegicus
3.1.3.2 2
-
 p-nitrophenyl phosphate
-
 Rattus norvegicus
3.1.3.2 2
-
 alpha-casein
-
 Rattus norvegicus
3.1.3.2 2.2
-
 GTP
-
 Rattus norvegicus
3.1.3.2 2.9
-
 thiamine diphosphate
-
 Rattus norvegicus
3.1.3.2 3.6
-
 4-methylumbelliferyl phosphate
-
 Rattus norvegicus
3.1.3.2 4.2
-
 phosvitin
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Fe2+ 0.1-1.0 mM, activation Rattus norvegicus
3.1.3.2 Iron contains 2 iron atoms per enzyme molecule Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 32000
-
 gel filtration Rattus norvegicus
3.1.3.2 33000
-
 1 * 33000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.2 glycoprotein
-
 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 spleen
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 106
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 1-naphthyl phosphate + H2O 37% of the activity with p-nitrophenyl phosphate Rattus norvegicus 1-naphthol + phosphate
-
 ?
3.1.3.2 2-glycerophosphate + H2O
-
 Rattus norvegicus glycerol + phosphate
-
 ?
3.1.3.2 4-methylumbelliferyl phosphate + H2O 82% of the activity with p-nitrophenyl phosphate Rattus norvegicus 4-methylumbelliferone + phosphate
-
 ?
3.1.3.2 ADP + H2O 102% of the activity with p-nitrophenyl phosphate Rattus norvegicus AMP + phosphate
-
 ?
3.1.3.2 alpha-casein + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.3.2 ATP + H2O 175% of the activity with p-nitrophenyl phosphate Rattus norvegicus ADP + phosphate
-
 ?
3.1.3.2 casein + H2O 9% of the activity with p-nitrophenyl phosphate Rattus norvegicus ?
-
 ?
3.1.3.2 CDP + H2O 69% of the activity with p-nitrophenyl phosphate Rattus norvegicus CMP + phosphate
-
 ?
3.1.3.2 CTP + H2O 130% of the activity with p-nitrophenyl phosphate Rattus norvegicus CDP + phosphate
-
 ?
3.1.3.2 diphosphate + H2O
-
 Rattus norvegicus 2 phosphate
-
 ?
3.1.3.2 Glucose 6-phosphate + H2O
-
 Rattus norvegicus Glucose + phosphate
-
 ?
3.1.3.2 GTP + H2O 125% of the activity with p-nitrophenyl phosphate Rattus norvegicus GDP + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Rattus norvegicus p-nitrophenol + phosphate
-
 ?
3.1.3.2 phenolphthalein diphosphate + H2O 67% of the activity with p-nitrophenyl phosphate Rattus norvegicus ?
-
 ?
3.1.3.2 phenolphthalein monophosphate + H2O 35% of the activity with p-nitrophenyl phosphate Rattus norvegicus phenolphthalein + phosphate
-
 ?
3.1.3.2 phenyl phosphate + H2O 52% of the activity with p-nitrophenyl phosphate Rattus norvegicus phenol + phosphate
-
 ?
3.1.3.2 phosphoenolpyruvate + H2O
-
 Rattus norvegicus pyruvate + phosphate
-
 ?
3.1.3.2 phosphopyruvate + H2O 22% of the activity with p-nitrophenyl phosphate Rattus norvegicus pyruvate + phosphate
-
 ?
3.1.3.2 phosvitin + H2O 15% of the activity with p-nitrophenyl phosphate Rattus norvegicus ?
-
 ?
3.1.3.2 thiamine diphosphate + H2O 76% of the activity with p-nitrophenyl phosphate Rattus norvegicus ?
-
 ?
3.1.3.2 UTP + 2 H2O 89% of the activity with p-nitrophenyl phosphate Rattus norvegicus UMP + 2 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 monomer 1 * 33000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5 5.8
-
 Rattus norvegicus