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Literature summary extracted from

  • Banno, Y.; Nozawa, Y.
    Purification and characterization of extracellular acid phosphatase of Tetrahymena pyriformis (1984), Biochim. Biophys. Acta, 799, 20-28.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 F-
-
 Tetrahymena pyriformis
3.1.3.2 Fe3+ 5 mM Tetrahymena pyriformis
3.1.3.2 Hg2+ 5 mM Tetrahymena pyriformis
3.1.3.2 molybdate noncompetitive Tetrahymena pyriformis
3.1.3.2 PCMB slight inhibition Tetrahymena pyriformis
3.1.3.2 Tartrate complete inhibition with 1 mM L-(+)-tartrate, no inhibition by D-(-)tartrate Tetrahymena pyriformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.31
-
 p-nitrophenyl phosphate
-
 Tetrahymena pyriformis
3.1.3.2 0.39
-
 adenosine 5'-phosphate
-
 Tetrahymena pyriformis
3.1.3.2 1.6
-
 glucose 6-phosphate
-
 Tetrahymena pyriformis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.2 extracellular
-
 Tetrahymena pyriformis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 61000
-
 2 * 61000, SDS-PAGE Tetrahymena pyriformis
3.1.3.2 120000
-
 gel filtration Tetrahymena pyriformis

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Tetrahymena pyriformis
-
-
-
3.1.3.2 Tetrahymena pyriformis W
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.2 glycoprotein carbohydrate content is about 10% of the total protein content Tetrahymena pyriformis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
 Tetrahymena pyriformis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 culture medium
-
 Tetrahymena pyriformis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 898.2
-
-
 Tetrahymena pyriformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 1-naphthyl phosphate + H2O
-
 Tetrahymena pyriformis 1-naphthol + phosphate
-
 ?
3.1.3.2 1-naphthyl phosphate + H2O
-
 Tetrahymena pyriformis W 1-naphthol + phosphate
-
 ?
3.1.3.2 5'-AMP + H2O
-
 Tetrahymena pyriformis adenosine + phosphate
-
 ?
3.1.3.2 5'-AMP + H2O
-
 Tetrahymena pyriformis W adenosine + phosphate
-
 ?
3.1.3.2 ADP + H2O
-
 Tetrahymena pyriformis AMP + phosphate
-
 ?
3.1.3.2 bis(p-nitrophenyl) phosphate + H2O no activity Tetrahymena pyriformis ?
-
 ?
3.1.3.2 D-fructose 1,6-diphosphate + H2O
-
 Tetrahymena pyriformis ?
-
 ?
3.1.3.2 D-fructose 1,6-diphosphate + H2O
-
 Tetrahymena pyriformis W ?
-
 ?
3.1.3.2 FMN + H2O
-
 Tetrahymena pyriformis ?
-
 ?
3.1.3.2 Glucose 6-phosphate + H2O
-
 Tetrahymena pyriformis Glucose + phosphate
-
 ?
3.1.3.2 Glucose 6-phosphate + H2O
-
 Tetrahymena pyriformis W Glucose + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Tetrahymena pyriformis p-nitrophenol + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Tetrahymena pyriformis W p-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 dimer 2 * 61000, SDS-PAGE Tetrahymena pyriformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 55
-
 half-life: 30 min Tetrahymena pyriformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 4.6
-
 hydrolysis of p-nitrophenyl phosphate, adenosine 5'-phosphate or glucose 6-phosphate Tetrahymena pyriformis