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Literature summary extracted from

  • Panara, F.; Pasqualini, S.; Antonielli, M.
    Multiple forms of barley root acid phosphatase: purification and some characteristics of the major cytoplasmic isoenzyme (1990), Biochim. Biophys. Acta, 1037, 73-80.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.2 EDTA 0.25-10 mM, slight activation Hordeum vulgare
3.1.3.2 Triton X-100 0.05-1.5%, slight activation Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 Ag+
-
 Hordeum vulgare
3.1.3.2 diethyl dicarbonate
-
 Hordeum vulgare
3.1.3.2 F- NaF Hordeum vulgare
3.1.3.2 Hg2+
-
 Hordeum vulgare
3.1.3.2 molybdate
-
 Hordeum vulgare
3.1.3.2 Pb2+
-
 Hordeum vulgare
3.1.3.2 phosphate competitive Hordeum vulgare
3.1.3.2 Urea 2.5 M, 50% inhibition Hordeum vulgare
3.1.3.2 Zn2+
-
 Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 additional information
-
 additional information pH-dependence of Km Hordeum vulgare
3.1.3.2 0.12
-
 p-nitrophenyl phosphate pH 4.8 Hordeum vulgare
3.1.3.2 0.19
-
 p-nitrophenyl phosphate pH 4.2 Hordeum vulgare
3.1.3.2 0.32
-
 p-nitrophenyl phosphate pH 4.0 Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.2 cytoplasm
-
 Hordeum vulgare 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 38000
-
 2 * 38000, SDS-PAGE Hordeum vulgare
3.1.3.2 77600
-
 gel filtration Hordeum vulgare
3.1.3.2 79000
-
 sucrose density gradient centrifugation Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Hordeum vulgare
-
 multiple enzyme forms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 major cytoplasmic isoenzyme Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 root
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 8.7
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 1-naphthyl phosphate + H2O 80% of the activity with p-nitrophenyl phosphate Hordeum vulgare 1-naphthol + phosphate
-
 ?
3.1.3.2 2-naphthyl phosphate + H2O 105% of the activity with p-nitrophenyl phosphate Hordeum vulgare 2-naphthol + phosphate
-
 ?
3.1.3.2 ADP + H2O 20% of the activity with p-nitrophenyl phosphate Hordeum vulgare AMP + phosphate
-
 ?
3.1.3.2 AMP + H2O 18% of the activity with p-nitrophenyl phosphate Hordeum vulgare adenosine + phosphate
-
 ?
3.1.3.2 ATP + H2O 75% of the activity with p-nitrophenyl phosphate Hordeum vulgare ADP + phosphate
-
 ?
3.1.3.2 glucose 1-phosphate + H2O 33% of the activity with p-nitrophenyl phosphate Hordeum vulgare glucose + phosphate
-
 ?
3.1.3.2 O-phospho-L-Tyr + H2O 62% of the activity with p-nitrophenyl phosphate Hordeum vulgare Tyr + phosphate
-
 ?
3.1.3.2 O-phosphoserine + H2O 48% of the activity with p-nitrophenyl phosphate Hordeum vulgare Ser + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Hordeum vulgare p-nitrophenol + phosphate
-
 ?
3.1.3.2 phenyl phosphate + H2O 80% of the activity with p-nitrophenyl phosphate Hordeum vulgare phenol + phosphate
-
 ?
3.1.3.2 phosphothreonine + H2O 25% of the activity with p-nitrophenyl phosphate Hordeum vulgare Thr + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 dimer 2 * 38000, SDS-PAGE Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 30 35
-
 Hordeum vulgare

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 40
-
 half-life: 40 min Hordeum vulgare
3.1.3.2 50
-
 half-life: 5 min Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5.2 6.8
-
 Hordeum vulgare