EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.2 | Cys | activates | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.2 | ATP | - |
Bos taurus | |
3.1.3.2 | Co2+ | competitive | Bos taurus | |
3.1.3.2 | diphosphate | - |
Bos taurus | |
3.1.3.2 | F- | competitive | Bos taurus | |
3.1.3.2 | molybdate | competitive | Bos taurus | |
3.1.3.2 | phosphate | competitive | Bos taurus | |
3.1.3.2 | Tartrate | tartrate has no effect | Bos taurus | |
3.1.3.2 | vanadate | competitive | Bos taurus | |
3.1.3.2 | Zn2+ | above 1 mM, uncompetitive | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.2 | 0.012 | - |
DL-phosphotyrosine | pH 7 | Bos taurus | |
3.1.3.2 | 0.014 | - |
p-nitrophenyl phosphate | pH 7 | Bos taurus | |
3.1.3.2 | 0.064 | - |
p-nitrophenyl phosphate | pH 8 | Bos taurus | |
3.1.3.2 | 0.083 | - |
DL-phosphotyrosine | pH 8 | Bos taurus | |
3.1.3.2 | 0.44 | - |
p-nitrophenyl phosphate | pH 9 | Bos taurus | |
3.1.3.2 | 0.45 | - |
DL-phosphotyrosine | pH 8 | Bos taurus | |
3.1.3.2 | 1.37 | - |
DL-phosphotyrosine | pH 9 | Bos taurus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.2 | Fe2+ | activates | Bos taurus | |
3.1.3.2 | Mn2+ | activates | Bos taurus | |
3.1.3.2 | Zn2+ | activates below 1 mM | Bos taurus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 32500 | - |
1 * 32500, SDS-PAGE | Bos taurus |
3.1.3.2 | 34600 | - |
gel filtration | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.2 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.2 | - |
Bos taurus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.3.2 | a phosphate monoester + H2O = an alcohol + phosphate | formation of a covalent phosphoryl-enzyme intermediate, kinetic pattern of a pseudo uni-bi hydrolytic two-step reaction mechanism | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.3.2 | bone | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 8.15 | - |
- |
Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.2 | ADP + H2O | at 12.9% of the activity with p-nitrophenyl phosphate | Bos taurus | AMP + phosphate | - |
? | |
3.1.3.2 | ATP + H2O | at 18.0% of the activity with p-nitrophenyl phosphate | Bos taurus | ADP + phosphate | - |
? | |
3.1.3.2 | CDP + H2O | at 6.6% of the activity with p-nitrophenyl phosphate | Bos taurus | CMP + phosphate | - |
? | |
3.1.3.2 | DL-phosphotyrosine + H2O | - |
Bos taurus | tyrosine + phosphate | - |
? | |
3.1.3.2 | GDP + H2O | at 18.5% of the activity with p-nitrophenyl phosphate | Bos taurus | GMP + phosphate | - |
? | |
3.1.3.2 | GTP + H2O | at 15.3% of the activity with p-nitrophenyl phosphate | Bos taurus | GDP + phosphate | - |
? | |
3.1.3.2 | O-phospho-L-Tyr + H2O | at 8.2% of the activity with p-nitrophenyl phosphate | Bos taurus | Tyr + phosphate | - |
? | |
3.1.3.2 | p-nitrophenyl phosphate + H2O | - |
Bos taurus | p-nitrophenol + phosphate | - |
? | |
3.1.3.2 | phosphotyrosyl histone + H2O | 4.2% of the activity with p-nitrophenyl phosphate | Bos taurus | histone + phosphate | - |
? | |
3.1.3.2 | TDP + H2O | at 10.6% of the activity with p-nitrophenyl phosphate | Bos taurus | TMP + phosphate | - |
? | |
3.1.3.2 | TTP + H2O | at 11.3% of the activity with p-nitrophenyl phosphate | Bos taurus | TDP + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.2 | monomer | 1 * 32500, SDS-PAGE | Bos taurus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 75 | - |
30 min, slight inactivation | Bos taurus |
3.1.3.2 | 90 | - |
rapid and irreversible inactivation | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 5.5 | - |
hydrolysis of p-nitrophenyl phosphate | Bos taurus |