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Literature summary extracted from

  • Lau, K.H.W.; Farley, J.R.; Baylink, D.J.
    Bone acid phosphatase is a neutral pH phosphoryl protein phosphatase (1987), Adv. Protein Phosphatases, 4, 165-198.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.2 Cys activates Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 ATP
-
Bos taurus
3.1.3.2 Co2+ competitive Bos taurus
3.1.3.2 diphosphate
-
Bos taurus
3.1.3.2 F- competitive Bos taurus
3.1.3.2 molybdate competitive Bos taurus
3.1.3.2 phosphate competitive Bos taurus
3.1.3.2 Tartrate tartrate has no effect Bos taurus
3.1.3.2 vanadate competitive Bos taurus
3.1.3.2 Zn2+ above 1 mM, uncompetitive Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.012
-
DL-phosphotyrosine pH 7 Bos taurus
3.1.3.2 0.014
-
p-nitrophenyl phosphate pH 7 Bos taurus
3.1.3.2 0.064
-
p-nitrophenyl phosphate pH 8 Bos taurus
3.1.3.2 0.083
-
DL-phosphotyrosine pH 8 Bos taurus
3.1.3.2 0.44
-
p-nitrophenyl phosphate pH 9 Bos taurus
3.1.3.2 0.45
-
DL-phosphotyrosine pH 8 Bos taurus
3.1.3.2 1.37
-
DL-phosphotyrosine pH 9 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Fe2+ activates Bos taurus
3.1.3.2 Mn2+ activates Bos taurus
3.1.3.2 Zn2+ activates below 1 mM Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 32500
-
1 * 32500, SDS-PAGE Bos taurus
3.1.3.2 34600
-
gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.2 a phosphate monoester + H2O = an alcohol + phosphate formation of a covalent phosphoryl-enzyme intermediate, kinetic pattern of a pseudo uni-bi hydrolytic two-step reaction mechanism Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 bone
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 8.15
-
-
Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 ADP + H2O at 12.9% of the activity with p-nitrophenyl phosphate Bos taurus AMP + phosphate
-
?
3.1.3.2 ATP + H2O at 18.0% of the activity with p-nitrophenyl phosphate Bos taurus ADP + phosphate
-
?
3.1.3.2 CDP + H2O at 6.6% of the activity with p-nitrophenyl phosphate Bos taurus CMP + phosphate
-
?
3.1.3.2 DL-phosphotyrosine + H2O
-
Bos taurus tyrosine + phosphate
-
?
3.1.3.2 GDP + H2O at 18.5% of the activity with p-nitrophenyl phosphate Bos taurus GMP + phosphate
-
?
3.1.3.2 GTP + H2O at 15.3% of the activity with p-nitrophenyl phosphate Bos taurus GDP + phosphate
-
?
3.1.3.2 O-phospho-L-Tyr + H2O at 8.2% of the activity with p-nitrophenyl phosphate Bos taurus Tyr + phosphate
-
?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
Bos taurus p-nitrophenol + phosphate
-
?
3.1.3.2 phosphotyrosyl histone + H2O 4.2% of the activity with p-nitrophenyl phosphate Bos taurus histone + phosphate
-
?
3.1.3.2 TDP + H2O at 10.6% of the activity with p-nitrophenyl phosphate Bos taurus TMP + phosphate
-
?
3.1.3.2 TTP + H2O at 11.3% of the activity with p-nitrophenyl phosphate Bos taurus TDP + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 monomer 1 * 32500, SDS-PAGE Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 75
-
30 min, slight inactivation Bos taurus
3.1.3.2 90
-
rapid and irreversible inactivation Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5.5
-
hydrolysis of p-nitrophenyl phosphate Bos taurus