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Literature summary extracted from

  • Zhou, S.; Clemens, J.C.; Hakes, D.J.; Barford, D.; Dixon, J.E.
    Expression, purification, crystallization, and biochemical characterization of a recombinant protein phosphatase (1993), J. Biol. Chem., 268, 17754-17761.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.16 expression in Escherichia coli Lambdavirus lambda

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.16
-
 Lambdavirus lambda

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.16 AMP
-
 Lambdavirus lambda
3.1.3.16 Co2+
-
 Lambdavirus lambda
3.1.3.16 Cr2+
-
 Lambdavirus lambda
3.1.3.16 Cu2+
-
 Lambdavirus lambda
3.1.3.16 Fe2+
-
 Lambdavirus lambda
3.1.3.16 Hg2+
-
 Lambdavirus lambda
3.1.3.16 Pd2+
-
 Lambdavirus lambda
3.1.3.16 phosphoenolpyruvate
-
 Lambdavirus lambda
3.1.3.16 Sc3+
-
 Lambdavirus lambda
3.1.3.16 tungstate
-
 Lambdavirus lambda
3.1.3.16 V3+
-
 Lambdavirus lambda
3.1.3.16 vanadate
-
 Lambdavirus lambda
3.1.3.16 Yb3+
-
 Lambdavirus lambda
3.1.3.16 Zn2+
-
 Lambdavirus lambda

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.16 25000
-
 SDS-PAGE Lambdavirus lambda

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Lambdavirus lambda
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.16 recombinant enzyme Lambdavirus lambda

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.16 3870
-
 substrate p-nitrophenyl phosphate Lambdavirus lambda

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 p-nitrophenyl phosphate + H2O
-
 Lambdavirus lambda p-nitrophenol + phosphate
-
 ?
3.1.3.16 phosphoproteins + H2O hydrolyzes phosphoseryl, phosphotyrosyl and phosphothreonyl substrates Lambdavirus lambda proteins + phosphate
-
 ?